At room T, sucrose is hydrolyzed by the catalytic action of the enzyme, sucrase. Starting with the sucrose concentration, CAO = 1 millimol/Li and a sucrase concentration, CB = 0.01 millimol/Li, the following kinetic data are obtained in a batch reactor: CA: millimol/Li t, hr 1 2 3 4 5 6 7 8 9 10 11 Determine whether these data can be reasonably fitted with a kinetic equation of the Michaelis-Menten type, or -TA 0.84 0.68 0.53 0.38 0.27 0.16 0.09 0.04 0.18 0.006 0.0025 = KCACB CA+M where M = Michaelis constant If the fit is reasonable, evaluate the rate constant, k, and M.

Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
Section: Chapter Questions
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At room T, sucrose is hydrolyzed by the catalytic action of the enzyme, sucrase.
Starting with the sucrose concentration, CA = 1 millimol/Li and a sucrase
concentration, CB = 0.01 millimol/Li, the following kinetic data are obtained in a
batch reactor:
CAS
millimol/Li
t, hr
1 2 3 4 5 6
7 8 9 10 11
Determine whether these data can be reasonably fitted with a kinetic equation of
the Michaelis-Menten type, or
-TA
0.84 0.68 0.53 0.38 0.27 0.16
=
0.09 0.04 0.18 0.006 0.0025
KCACB
CA+M
where M = Michaelis constant
If the fit is reasonable, evaluate the rate constant, k, and M.
Transcribed Image Text:At room T, sucrose is hydrolyzed by the catalytic action of the enzyme, sucrase. Starting with the sucrose concentration, CA = 1 millimol/Li and a sucrase concentration, CB = 0.01 millimol/Li, the following kinetic data are obtained in a batch reactor: CAS millimol/Li t, hr 1 2 3 4 5 6 7 8 9 10 11 Determine whether these data can be reasonably fitted with a kinetic equation of the Michaelis-Menten type, or -TA 0.84 0.68 0.53 0.38 0.27 0.16 = 0.09 0.04 0.18 0.006 0.0025 KCACB CA+M where M = Michaelis constant If the fit is reasonable, evaluate the rate constant, k, and M.
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