Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
Suppose that you want to sequence the following DNA fragment:
5′–TCCCGGGAAA-primer site–3′
You first use PCR to amplify the fragment, so that there is sufficient
DNA for sequencing. You carry out dideoxy sequencing and then
separate the products of the sequencing reactions by gel electrophoresis.
Draw the bands that should appear on the gel from the four sequencing
reactions.
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