Question 2. It is your first week working in the lab but unfortunately you program the PCR machine incorrectly and some of your PCR experiments do not work as expected. For each of the following scenarios, state at which step the PCR went wrong, what was wrong and what would have happened. Cycles Which step is incorrect? Is the temp too high or too low? Experiment of What happened? PCR Step 1 94 °C Step : 94°C V ( Select ] [ Select ] ( Select] a step 2 Step 3 72 °C step 3 step 1 Step 1 30 °C Step 2 60 °C ( Select ] ( Select ] [ Select ] Step 3 72 °C Step 1 94 °C ( Select ) Step 2 22°C [ Select ] ( Select ) Step 3 72 °C <>
Question 2. It is your first week working in the lab but unfortunately you program the PCR machine incorrectly and some of your PCR experiments do not work as expected. For each of the following scenarios, state at which step the PCR went wrong, what was wrong and what would have happened. Cycles Which step is incorrect? Is the temp too high or too low? Experiment of What happened? PCR Step 1 94 °C Step : 94°C V ( Select ] [ Select ] ( Select] a step 2 Step 3 72 °C step 3 step 1 Step 1 30 °C Step 2 60 °C ( Select ] ( Select ] [ Select ] Step 3 72 °C Step 1 94 °C ( Select ) Step 2 22°C [ Select ] ( Select ) Step 3 72 °C <>
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Question 2. It is your first week working in the lab but unfortunately you program the PCR machine incorrectly and some of your PCR experiments do
not work as expected. For each of the following scenarios, state at which step the PCR went wrong, what was wrong and what would have happened.
Cycles
Which step is incorrect?
Is the temp too high or too
low?
Experiment of
What happened?
PCR
Step 1
94 °C
Step
94°C
V [ Select ]
[ Select ]
[ Select ]
a
step 2
Step 3
72 °C
step 3
step 1
Step 1
30 °C
Step 2
60 °C
[ Select )
[ Select ]
[ Select ]
Step 3
72 °C
Step 1
94 °C
[ Select]
Step 2
22°C
[ Select ]
[ Select ]
Step 3
72 °C
8
A átv](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fb03e62df-3e10-4baf-bf86-6136e47c190d%2F834dba7b-3771-4339-996b-672a942acd1a%2Ftjbxug_processed.jpeg&w=3840&q=75)
Transcribed Image Text:Instructure.com/courses/47420/quizzes/225324/take
missour
Question 2. It is your first week working in the lab but unfortunately you program the PCR machine incorrectly and some of your PCR experiments do
not work as expected. For each of the following scenarios, state at which step the PCR went wrong, what was wrong and what would have happened.
Cycles
Which step is incorrect?
Is the temp too high or too
low?
Experiment of
What happened?
PCR
Step 1
94 °C
Step
94°C
V [ Select ]
[ Select ]
[ Select ]
a
step 2
Step 3
72 °C
step 3
step 1
Step 1
30 °C
Step 2
60 °C
[ Select )
[ Select ]
[ Select ]
Step 3
72 °C
Step 1
94 °C
[ Select]
Step 2
22°C
[ Select ]
[ Select ]
Step 3
72 °C
8
A átv
![Question 2. It is your first week working in the lab but unfortunately you program the PCR machine incorrectly and some of your PCR experiments do
not work as expected. For each of the following scenarios, state at which step the PCR went wrong, what was wrong and what would have happened.
Cycles
Which step is incorrect?
Is the temp too high or too
low?
Experiment of
What happened?
PCR
Step 1
94 °C
Step 2
94°C
[ Select ]
[ Select ]
v v [ Select ]
a
Primers will anneal in the wrong places
Step 3
72 °C
DNA won't denature
Primers won't anneal
Step 1
30 °C
Step 2
60 °C
[ Select ]
[ Select ]
[ Select ]
Step 3
72 °C
Step 1
94 °C
Step 2
22°C
[ Select ]
[ Select ]
[ Select ]
Step 3
72 °C](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fb03e62df-3e10-4baf-bf86-6136e47c190d%2F834dba7b-3771-4339-996b-672a942acd1a%2Fedso3xc_processed.jpeg&w=3840&q=75)
Transcribed Image Text:Question 2. It is your first week working in the lab but unfortunately you program the PCR machine incorrectly and some of your PCR experiments do
not work as expected. For each of the following scenarios, state at which step the PCR went wrong, what was wrong and what would have happened.
Cycles
Which step is incorrect?
Is the temp too high or too
low?
Experiment of
What happened?
PCR
Step 1
94 °C
Step 2
94°C
[ Select ]
[ Select ]
v v [ Select ]
a
Primers will anneal in the wrong places
Step 3
72 °C
DNA won't denature
Primers won't anneal
Step 1
30 °C
Step 2
60 °C
[ Select ]
[ Select ]
[ Select ]
Step 3
72 °C
Step 1
94 °C
Step 2
22°C
[ Select ]
[ Select ]
[ Select ]
Step 3
72 °C
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