Problem 11 of 15SubmitUsing the following reaction datapoints, construct a Lineweaver-Burkplot for an enzyme with and without anoncompetitive inhibitor by draggingthe points to their relevantcoordinates on the graph and drawinga line of best fit.with 10 μg per mL inhibitor1-1s mMV'7148<363027Answer:||| Problem 11 of 15SubmitUsing the following reaction datapoints, construct a Lineweaver-Burkplot for an enzyme with and without anoncompetitive inhibitor by draggingthe points to their relevantcoordinates on the graph and drawinga line of best fit.1-1 1mM[S]'20V's mM¹ with 10 μg per541036>ст5272.5231.2520Answer:|||

III. Interpretation of the Lineweaver-Burk Plot1. Effect of the Noncompetitive InhibitorThe two…

Answered: Problem 11 of 15 Submit Using the…

Biochemistry

9th Edition

ISBN:9781305961135

Author:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal

Publisher:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal

Chapter6: The Behavior Of Proteins: Enzymes

Section: Chapter Questions

Problem 60RE

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MATHEMATICAL If a reaction can be written AB, and the G is 20kJmol1, what would the substrate/product ratio have to be for the reaction to be thermodynamically favorable?
MATHEMATICAL You do an enzyme kinetic experiment and calculate a Vmax of 100 mol of product per minute. If each assay used 0.1 mL of an enzyme solution that had a concentration of 0.2 mg/mL, what would be the turnover number if the enzyme had a molecular weight of 128,000 g/mol?
MATHEMATICAL The standard free-energy change for the reaction Arginine+ATPPhosphoarginine+ADP is +1.7kJmol1. From this information and that in Table 15.1, calculate the G for the reaction Phosphoarginine+H2OArginine+Pi
MATHEMATICAL For the Vmax obtained in Question 26, calculate the turnover number (catalytic rate constant) assuming that 131024mol of enzyme were used.
The energy released by the hydrolysis of ATP is primarily stored between the alpha and beta phosphates equal to -57 kcal/mol harnessed as heat energy by the cell to perform work providing energy to coupled reactions
MATHEMATICAL The kinetic data in the following table were obtained for the reaction of carbon dioxide and water to produce bicarbonate and hydrogen ion catalyzed by carbonic anhydrase: CO2+H2OHCO3+H+ [H. De Voe and G. B. Kistiakowsky, J. Am. Chem. Soc. 83, 274 (1961)]. From these data, determine KM and Vmax for the reaction. CarbonDioxideconcentration(mmolL1)1/Velocity(M1sec)1.252.55.020.00.25036103201031210361030.256
MATHEMATICAL The free-energy change (G) for the oxidation of the cytochrome aa3 complex by molecular oxygen is 2102.3kJ=224.5kcal for each mole of electron pairs transferred. What is the maximum number of moles of ATP that could be produced in the process? How many moles of ATP are actually produced? What is the efficiency of the process, expressed as a percentage?
MATHEMATICAL Consider the reaction AB+C, where G=0.00. (a) What is the value of G (not G) when the initial concentrations of A, B, and C are 1 M, 103M,and106M? (b) Try the same calculations for the reaction D+EF, for the same relative order of concentrations. (c) Try the same calculations for the reaction GH, if the concentrations are 1Mand103M for G and H, respectively.
MATHEMATICAL The enzyme -methylaspartase catalyzes the deamination of -methylaspartate [V. Williams and J. Selbin, J. Biol. Chem. 239, 1636 (1964)]. The rate of the reaction was determined by monitoring the absorbance of the product at 240 nm(A240). From the data in the following table, determine KM for the reaction. How does the method of calculation differ from that in Questions 26 and 27?
MATHEMATICAL The G for the reaction Citrate Isocitrare is +6.64kJmol1=+1.59kcalmol1. The G for the reaction Isocitrate -Ketoglutrate is 267kJmol1=63.9kcalmol1. What is the G for the conversion of citrate to - ketoglutarate? Is that reaction exergonic or endergonic, and why?
MATHEMATICAL Calculate the net ATP yield from linoleic acid (18:29,12). For this calculation, assume that the loss of an NADPH is the same as the loss of an NADH.
In each of the three systems, determine the state of entropy (low or high) when comparing the first and second: i. the instant that a perfume bottle is sprayed compared with 30 seconds later, ii. an old 1950s car compared with a brand new car, and iii. a living cell compared with a dead cell. a. i. low, ii. high, iii. low b. i. low, ii. high, iii. high c. i. high, ii. low, iii. high d. i. high, ii. low, iii. low

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