Phosphoric acid is a triprotic acid and the phosphate buffer system is used to buffer cells around 6.9 – 7.4. H3PO4(s) + H2O(1) -> H3O+ (aq) + H₂PO4 (aq) H2PO4(aq)+ H2O(1) -> H3O+ (aq) + HPO4²¯ (aq) HPO4²¯ (aq) + H2O(1) -> H3O+ (aq) + PO4³¯ (aq) Kai= 7.25×10¯³ Ka2= 6.31×10-8 Ka3 = 4.80×10¯13
Phosphoric acid is a triprotic acid and the phosphate buffer system is used to buffer cells around 6.9 – 7.4. H3PO4(s) + H2O(1) -> H3O+ (aq) + H₂PO4 (aq) H2PO4(aq)+ H2O(1) -> H3O+ (aq) + HPO4²¯ (aq) HPO4²¯ (aq) + H2O(1) -> H3O+ (aq) + PO4³¯ (aq) Kai= 7.25×10¯³ Ka2= 6.31×10-8 Ka3 = 4.80×10¯13
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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1. Sketch the general shape of the titration curve for the addition of hydroxide to phosphoric acid all the way to phosphate.
2. Given the cellular pH, which chemical species are present in the cell? Label the relevant weak acid and conjugate base
3. For the relevant equilibrium present in the cell, sketch the titration curve. Make sure the following are labeled:
- The x and y axes.
- The relevant pKa.
- The endpoint.
- The buffer zone.
- The point at which there is a 50/50 mixture of weak acid and conjugate base.
- The relative amounts of the weak acid and conjugate base (use the specific chemical species you determined in part 3) along each segment of the titration curve
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