Please indicate true or false about the statements regarding flies with the following genotype: Hs>FLP; FRT82B, Actin-GFP, e2f1i2. e2f1i2 is a mutation and that both this mutation and Actin-GFP reporter are located on the same chromosomal arm as FRT82B. This line can be crossed with w; FRT82B to create mosaic clones in wing discs after heat shock. Cells with higher GFP intensity correspond to the WT and the lower intensity GFP-positive cells are heterozygous. When this line was crossed with w; FRT82B males, no mosaic clones will be generated in wing discs even after heat shock. All the cells in wing discs will have similar level of GFP expression. This line can be crossed with w; FRT82B to create mosaic clones in wing discs after heat shock. Cells with no GFP expression can be used to identify the WT cells. This line can be crossed with w; FRT82B to create mosaic clones in wing discs after heat shock. Cells with no GFP expression can be used to identify the e2f1i2 mutant cells.

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Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Please indicate true or false about the statements regarding flies with the following genotype: Hs>FLP; FRT82B, Actin-GFP, e2f1ⁱ². e2f1ⁱ² is a mutation and that both this mutation and Actin-GFP reporter are located on the same chromosomal arm as FRT82B.

This line can be crossed with w; FRT82B to create mosaic clones in wing discs after heat shock. Cells with higher GFP intensity correspond to the WT and the lower intensity GFP-positive cells are heterozygous.

When this line was crossed with w; FRT82B males, no mosaic clones will be generated in wing discs even after heat shock. All the cells in wing discs will have similar level of GFP expression.

This line can be crossed with w; FRT82B to create mosaic clones in wing discs after heat shock. Cells with no GFP expression can be used to identify the WT cells.

This line can be crossed with w; FRT82B to create mosaic clones in wing discs after heat shock. Cells with no GFP expression can be used to identify the e2f1ⁱ²mutant cells.

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