Now, figure out the complementation groups. This shows how many genes are involved in this pathway. Some of the mutant classes in the previous problem must contain more than one complementation group (gene) For example class 2 is defined by mutants 1,3,4,6, and 7. These mutants may, or may not, all be in the same gene. Complementation testing will help you know if they are, or are not, in the same gene. So there are actually additional steps between some of the letters. For example, maybe instead of XàY, it is really XàFàGàY. (c) The singiCHALLENGE PROBLEM 2 You are carrying out BeadleTatum type experiments to analyze a metabolic pathway inNeurospora. You know that the precursor in the pathway is amolecule symbolized as P and that the product is a vitaminsymbolized as Z. You are sure that the pathway from P to Zislinear and that the molecules W, X, and Y are intermediates.However, there may be other intermediates not yet identi-fied. You obtain 10 independent mutations that cannot growon minimal medium supplemented with P, but can grow onminimal medium supplemented with Z. The 10 mutants fallinto four classes that can grow (+) or cannot grow (-) onminimal medium supplemented with the nutrients W, X,or Y. The data are shown in the accompanying table.P W XY ZClass I (mutant 5)Class II (mutants 1, 3. 4. 6, 7)Class III (mutant 2)Class IV (mutants 8, 9, 10)Draw a linear metabolic pathway with P on the left and Zon the right, in which each of the intermediates W, X, andY is shown in the order in which it occurs in the metabolicpathway in the synthesis of Z from P. CHALLENGE PROBLEM 3 The 10 mutants in ChallengeProblem 2 were tested for complementation in all pairwisecombinations using heterokaryons. The results are shownin the matrix, in which + indicates the ability of the hetero-karyon to grow in minimal medium and – indicates inabil-ity to grow in minimal medium.2 3 4 5 6 7 89.10Mutant 1+ ++ + +3.+ +++ ++ +6.+ +78.6.Assume that each complementation group defines adifferent gene, and assume further that each geneencodes an enzyme that catalyzes a single step in themetabolic pathway, which converts one molecule ofsubstrate into one molecule of product.(a) Redraw the metabolic pathway deduced from Chal-lenge Problem 2. Use a right arrow to indicate eachenzymatic step in the pathway, and label each arrowwith the mutant number 1-10 that blocks the enzy-matic step. In some cases, you will not be able to specifythe order in which the enzymes occur in the pathway,so you may write them in any order you wish.(b) In the metabolic pathway that you have deduced fromthe data, how many unknown intermediates are therebetween the precursor P and the vitamin Z?+ + ++ + +十 ++ +2.

Name: Now, figure out the complementation groups. This shows how many genes
Uploaded: 2024-03-20T06:42:55.000Z
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Description: Now, figure out the complementation groups. This shows how many genes are involved in this pathway. Some of the mutant classes in the previous problem must contain more than one complementation group (gene) For example class 2 is defined by mutants 1