plasmid has a total size of 2686 bp. There is an EcoRI recognition site at position 439, BamHI recognition site at position 447, and SmaI recognition site at 2179. If one restriction enzyme will be used to cut this plasmid, predict the fragment type and size that it would produce: a. circular 2179bp plasmid b. one 447bp fragment and 439bp fragment c. one 1800bp fragment and 886bp fragment or d. a linear 2686bp plasmid
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
A plasmid has a total size of 2686 bp. There is an EcoRI recognition site at position 439, BamHI recognition site at position 447, and SmaI recognition site at 2179. If one restriction enzyme will be used to cut this plasmid, predict the fragment type and size that it would produce:
a. circular 2179bp plasmid
b. one 447bp fragment and 439bp fragment
c. one 1800bp fragment and 886bp fragment
or
d. a linear 2686bp plasmid
Step by step
Solved in 2 steps