Find a plasmid map for pET11a and create a basic procedure for cloning a gene into this vector. Which selection method and substance would you use for this plasmid after transformation?

Q: an insert check (labeled A-D below). After growing cultures, you isolate the plasmids and treat them…

A: In E. Coli lac operon contains a structural gene lac Z that codes for the enzyme beta galactosidase.…

Q: Colonies/ug of plasmid) based on these results? O about 140O O about 800 O about 4000 O about 400…

A: INTRODUCTION Transformation efficiency is that the efficiency by which cells can take up…

Q: pZERO®-1. Some vectors are designed specifically for TA cloning while some are not. What is TA…

A: A vector is a living organism that can transmits an infectious agent from an infected animal to…

Q: The plasmids from the pUC series are created in the University of California. They carry a lacZ gene…

A: The pUC series plasmids have a lacZ gene that act as a selectable marker. The lacZ gene contains the…

Q: nion and burger lover, your friend would like to incorporate the cheems gene (“cheM”) from the rare…

A: Transformation is a process in which the original DNA is transformed by the addition of foreign DNA…

Q: For pGLO plasmid that contains araC gene and Ampr gene, what are the names of their promoters and…

A: pGLO plasmid  pGLO plasmid is a 5371bp genetically engineered plasmid to create genetically modified…

Q: Plasmid A carries an ampicillin resistant gene (bla) and a gene for production of a GFP protein;…

A: Plasmids are important tools for modifying and analyzing genes in genetic engineering and molecular…

Q: List the major steps that would be required to clone the human insulin gene into a plasmid and to…

A: Gene cloning is the isolation of DNA (deoxyribonucleic acid) sequence or gene of interest and…

Q: This enzyme will be added when the cells are lysed, detroying all RNA in the sample. Why will this…

A: INTRODUCTION Plasmid Plasmid are circular DNA and into which we can insert the DNA of our interest…

Q: What does the F plasmid code for?

A: The plasmid is defined as a small, circular, extra-chromosomal, double-stranded DNA molecule present…

Q: A) Outline the experimental procedure for cloning a eukaryotic gene and expressing it in E. coli.…

A: Genetic engineering has enabled us to engineer the genes according to the desired gene product. It…

Q: Construct a restriction map for Plasmid X to show the recognition sites for two commonly used…

A: A restriction map is a representation of known restriction sites in a DNA sequence. Restriction…

Q: If I clone a complete eukaryotic gene, including the eukaryotic promoter region, ligate it into a…

A: Transformation is the process in which a bacterium takes up DNA from the environment, that has been…

Q: Most bacteria which are used in molecular biology are ampicillin sensitive and recA. Plasmid usually…

A: Mostly ampicillin sensitive and recA- bacteria are used in molecular biology for the transformation.…

Q: A plasmid vector has two genes in it, a gene for penicillin resistance (penR) and another for…

A: "Biotechnology" is the use of our knowledge of biological processes to the development of beneficial…

Q: What is blue/white screening? What is the key feature of a plasmid that is used for it?

A: The blue-white screen is a screening technique that allows for the rapid and convenient detection of…

Q: You are using Drosophila as a model system to investigate eye development. You do positional cloning…

A: In order to isolate a mutant gene in any organism, one would need to first identify the desired…

Q: Why are nucleic acid probes necessary to lyse cells containing plasmids in order to detect the…

A: Cell lysis is frequently the very first step in most purification techniques for nucleic acids,…

Q: You want to express the protein of a newly discovered human gene using E. coli, so you clone the…

A: Protein tags are most frequently used to purify proteins for which no protein-specific antibody…

Q: С 17 Based on the knowledge you gained from the cloning module, which of the bands in the figure is…

A: Agarose gel electrophoresis is a method used in molecular biology to resolve DNA fragments on the…

Q: Some expression plasmids have small peptide or protein tags in front of and/or after the cloning…

A: An expression plasmid or expression vector is a plasmid which is designed for gene expression in…

Q: Name any two cloning vectors. Describe the features required to facilitate cloning into a vector.

A: Cloning is a process that allows scientists to create perfect genetic duplicates of living…

Q: Briefly describe two different methods for inserting foreign DNA into plasmids, giving the strengths…

A: The plasmid is typically a small circular deoxyribonucleic acid (DNA) strand in the cytoplasm of the…

Q: A newly discovered insect has a genomic DNA that does not contain any repetitive DNA or any intronic…

A:

Q: plasmid has been modified such that it carries the gene for green fluorescent protein (GFP) under…

A: GFP protein is found in the photoorgans of Aequorea Victoria a jelly fish. It is a 28 kDa protein.…

Q: which parts would a plasmid vector with 2500 bp have in E. coli

A: A plasmid is a small, circular, double-stranded DNA molecule that is distinct from a cell's…

Q: Discuss the process and principle involved for screening/selection of hosts (last stage of cloning)…

A: Molecular cloning or cloning is a technique to assemble DNA of two different organisms and directing…

Q: Explain the THREE (3) important regions of this plasmid that enable it to work efficiently as a…

A: Plasmid is a self replicating generally circular DNA molecule that can be used to carry a foreign…

Q: For the plasmid below, list the origin and what antibiotic you would use for selection?

A: Plasmid is the extra chromosomal DNA present in prokaryotic cells. It contains information that…

Genetic Evolution

Sexual reproduction produces distinct population variations. Heredity determines the traits and qualities to be passed down to the progeny. Over time, this variation within-species is also affected by the process of human evolution. The study of how genetic variation leads to evolutionary changes is known as genetic evolution. If a trait helps an organism to survive and reproduce, it is more likely to be passed down the generations. It is rare that such traits result in a completely distinct set of traits in descendants than the ancestral population; this can result in the emergence of a new species.