Partial hydrolysis of the salivary enzyme yielded the hexapeptide RMKEFM. 8. Calculate the pl of the polypeptide. Show your solutior 9. Show the different species present at different pH. 10. What are the two predominant species at pH 5? 11. What are relative % abundance of the species in the previous question? Show your solution.
Partial hydrolysis of the salivary enzyme yielded the hexapeptide RMKEFM. 8. Calculate the pl of the polypeptide. Show your solutior 9. Show the different species present at different pH. 10. What are the two predominant species at pH 5? 11. What are relative % abundance of the species in the previous question? Show your solution.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Transcribed Image Text:Partial hydrolysis of the salivary enzyme yielded the hexapeptide RMKEFM.
8. Calculate the pl of the polypeptide. Show your solutior
9. Show the different species present at different pH.
10. What are the two predominant species at pH 5?
11. What are relative % abundance of the species in the previous question? Show your solution.
Rel Hyd* Time buried
in proteln
Amino Acid
3-
1-
pK1
pK2
pK3
letter
abbrev abbrev
Ala
Arg
AsN
letter
Alanine
Arginine
Asparagine
Aspartic acid
Cysteine
A
2.34
9.69
0.5
0.36
R
2.17
9.04
12.48
-11.2
0.01
0.12
8.80
9.82
N
2.02
-0.2
Asp
Cys
D
2.09
3.86
-7.4
0.15
C
1.71
-2.8
10.78
9.67
8.33
4.25
0.40
Glutamic acid
-9.9
Glu
GIN
Gly
His
2.19
0.18
Glutamine
Q
G
2.17
9.13
-0.3
0.07
Glycine
Histidine
2.34
0.38
0.17
9.60
H
1.82
9.17
6.00
-0.5
Isoleucine
lle
2.36
9.68
2.5
1.8
4.2
1.3
0.6
Leucine
Leu
L
2.36
9.68
0.45
10.53
Lysine
Methionine
Phenylalanine
Proline
Serine
Threonine
Tryptophan
Тугosine
Valine
0.03
0.40
Lys
K
2.18
8.95
9.21
9.13
Met
2.28
Phe
F
1.83
2.5
0.50
-3.3
-0.3
Pro
P
1.99
10.60
0.18
Ser
S
2.21
9.15
0.22
Thr
2.63
10.43
0.4
0.23
Trp
Туr
Val
W
2.38
9.39
3.4
0.27
Y
2.20
9.11
10.07
2.3
0.15
V
2.32
9.62
1.5
0.54
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