O Ara (Alt+ Ğ)Yeni. -Gözden GeçirGörünümYardımBaşvurularPosta Gönderileri1) Discuss this graph in the meaning of substrate depletion.10080[S][P]60402020406080100Time (s.)ere goz atın7SUS171958.9.Relative Concentration

Substrate: S Substrate concentration: [S] Product : P Substrate concentration : Becomes depleted…

Answered: 1) Discuss this graph in the meaning of…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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SUBSTRATE CONCENTRATION [S] µM INITIAL VELOCITY V0 s-1 10 0.13 25 0.27 50 0.45 100 0.65 150 0.77 200 0.85 300 0.94 500 1.03 (i) a) Construct an empty table with the following column headings: Substrate concentration [S] and initial velocity (Vi) where [S] has the unit µM, and Vi has the unit mM/s. (ii) The table provided is the enzyme kinetic data for your mutated enzyme, whereby Vi was expressed using the unit ∆A(405 nm)/s. Using the standard curve, express Vi with the unit mM/s rather than ∆A(405 nm)/s. Place your answer in the table above alongside the appropriate [S]. Hint: To answer this question you need to use this standard curve equation=0.0419x (The slope of the line is= 0.0419) (iii) The unmutated form of your protein has a Km of 25 µM and a Vmax of 43 mM/s. The enzyme kinetic data for your enzyme with the amino acid substitution should now be displayed in the table above. Based on these data, what is Vmax? Km? and…
Km of LDH with substrate 1 is 60 M and Km with substrate 2 is 45 M. The enzyme has a higher affinity for substrate 2. true or false
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A person (70kg) eating locally caught fish from water contaminated with 100ppb TCE and 100ppb aniline. The bioconcentration value for TCE and aniline in fish is the same at 1.06mg TCE (or aniline) per kg fish, respectively. Fish is only exposed to these contaminants in its environment. About 54g of fish is consumed in 350days/yr for 30years. Assume bioavailability value of 1. The potency factor for TCE is 1.1 × 10$# kg.day/mg and reference dose is 1.00 × 10$% mg/kg.day. Whereas the potency factor for aniline is 5.7 × 10$* kg.day/mg and reference dose (based on drinking water) is 1.60 × 10$& µg/L. Unlike aniline, TCE is not a carcinogenic compound. There are 70yrs in a lifetime and 365day/yr. a) Using chronic daily intake calculation, determine the non-cancer risk (number of people in one million) for the above case. b) Calculate the cancer risk (number of people in million) for the above case.
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What is the initial velocity measurement for the hydrolysis of 50 μM amoxicillin by the enzyme?
The hydrolysis of a substrate, S, by an enzyme has been studied in the lab. The following initial rates, vo, were recorded at different concentrations. [S] (M) Vo (M/min) 2.10-10-4 1.20.10-6 4.20-10-4 3.10.10-6 9.30-10-4 6.30-10-6 1.42-10-3 9.10-10-6 A. Determine the rate constants for degradation of the substrate B. What is the rate of reaction at [S] = 1.1.104 M? C. Explain, why enzymes can make reactions go faster? Does enzymes also catalyse the reverse reaction from product to substrate?
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● • 24) Determine the fraction of Vmax that would be found at a substrate concentration of ½2 Km, 2 Km and 10 Km.

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1) Discuss this graph in the meaning of substrate depletion. 100 80 [P] 60 40 20 20 40 60 80 100 Time (s.) Relative Concentration