Homework Help is Here – Start Your Trial Now!
Science
Biochemistry
2. Peter does not tolerate lactose in milk. He bought a solution of ß-galactosidase where [Eo] 10.0 µM. The concentration of lactose in milk is 150 mM and pH=6.3 Enzyme activity depends on pH with constants: pka1-6.0, pKa2=7.3 (the active form is partially protonated), KM-70 mM, kcat = 525 s¹ and enzyme loses half of its activity in 8 h. Find much enzyme solution (in mL) should Peter add to 1L of milk to reduce the concentration of lactose by 80% after 10h to maximize the enzyme usage efficiency.

2. Peter does not tolerate lactose in milk. He bought a solution of ß-galactosidase where [Eo] 10.0 µM. The concentration of lactose in milk is 150 mM and pH=6.3 Enzyme activity depends on pH with constants: pka1-6.0, pKa2=7.3 (the active form is partially protonated), KM-70 mM, kcat = 525 s¹ and enzyme loses half of its activity in 8 h. Find much enzyme solution (in mL) should Peter add to 1L of milk to reduce the concentration of lactose by 80% after 10h to maximize the enzyme usage efficiency.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
See similar textbooks
icon
Related questions
Question
Please give me correct solution.
2. Peter does not tolerate lactose in milk. He bought a solution of B-galactosidase where [Eo] 10.0 µM. The concentration of lactose in milk is 150 mM and pH=6.3 Enzyme activity depends on pH with constants: pka1-6.0, pka2=7.3 (the active form is partially protonated), KM-70 mm, kcat = 525 s¹ and enzyme loses half of its activity in 8 h. Find much enzyme solution (in mL) should Peter add to 1L of milk to reduce the concentration of lactose by 80% after 10h to maximize the enzyme usage efficiency.
Transcribed Image Text:2. Peter does not tolerate lactose in milk. He bought a solution of B-galactosidase where [Eo] 10.0 µM. The concentration of lactose in milk is 150 mM and pH=6.3 Enzyme activity depends on pH with constants: pka1-6.0, pka2=7.3 (the active form is partially protonated), KM-70 mm, kcat = 525 s¹ and enzyme loses half of its activity in 8 h. Find much enzyme solution (in mL) should Peter add to 1L of milk to reduce the concentration of lactose by 80% after 10h to maximize the enzyme usage efficiency.
Expert Solution
steps

Step by step

Solved in 2 steps

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON
SEE MORE TEXTBOOKS