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Write the enzyme model for chymotrypsin acting on PNPA. Which step must be the slowest in order to see pre-steady state burst kinetics? Why did you not see a burst of product formation in the experiment described above?

Write the enzyme model for chymotrypsin acting on PNPA. Which step must be the slowest in order to see pre-steady state burst kinetics? Why did you not see a burst of product formation in the experiment described above?

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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You mix together a little bit of chymotrypsin and some PNPA in a cuvette and start to see the yellow product being formed. You then place the cuvette in your trusty spectrophotometer and measure the increase in absorbance to calculate rate of product formation. In the data you collect, you do not see any burst, just a steady level of product formation. 4. a) Write the enzyme model for chymotrypsin acting on PNPA. Which step must be the slowest in order to see pre-steady state burst kinetics? b) Why did you not see a burst of product formation in the experiment described above?
Transcribed Image Text:You mix together a little bit of chymotrypsin and some PNPA in a cuvette and start to see the yellow product being formed. You then place the cuvette in your trusty spectrophotometer and measure the increase in absorbance to calculate rate of product formation. In the data you collect, you do not see any burst, just a steady level of product formation. 4. a) Write the enzyme model for chymotrypsin acting on PNPA. Which step must be the slowest in order to see pre-steady state burst kinetics? b) Why did you not see a burst of product formation in the experiment described above?
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