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How to prepare Figure 1:. (Refer to Postlab photo of gel for an example.

How to prepare Figure 1:. (Refer to Postlab photo of gel for an example.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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How I prepárate this figure ? Please I’m really stuck and I need to understand how to do. It
esc How to prepare Figure 1:. (Refer to Postlab photo of gel for an example. 1 of 1 ! 1 Q A. CROP YOUR GEL TO REMOVE EDGES OF PICTURE B. Add a Label to the top of your gel with lane titles C. Type the MW of the ladder bands along the side of the gel. D. (Hint: Click on Gel, Click on "Position" menu, Choose "more layout options". Place photo behind text. Now you can type along the top and side of the gel easily.) @ 2 W #3 Caption E $ 4 R % 5 T MacBook Pro 6 Y & 7 U * 00 8
Transcribed Image Text:esc How to prepare Figure 1:. (Refer to Postlab photo of gel for an example. 1 of 1 ! 1 Q A. CROP YOUR GEL TO REMOVE EDGES OF PICTURE B. Add a Label to the top of your gel with lane titles C. Type the MW of the ladder bands along the side of the gel. D. (Hint: Click on Gel, Click on "Position" menu, Choose "more layout options". Place photo behind text. Now you can type along the top and side of the gel easily.) @ 2 W #3 Caption E $ 4 R % 5 T MacBook Pro 6 Y & 7 U * 00 8
a travelled by the dye front. DYE FRONT S mm 4. Measure in mm (not cm) the distance travelled (or "migration distance") for each protein in your ladder. Measure from the bottom of the well to each band. 5. Use the Excel Tutorial Video on graphing and solving for the kD sizes of the unknowns. Protein Ladder (for trendline graph): Migration Distance (mm) Rf= Band Migration Dye Front Migration 0-074 0-111 d 17 22, 28. 31 36 Measurements for Unknown A Migration Distance (mm) 4 0-166 0.203 0.314. 0-407 esig 0.574 0,759, ما 14 Measurements for Unknown Migration Distance (mm) 17 Molecular Weight (in KD) 260 250 Rf •074 A Rf 315 JUSLY 20 10 1 kDa --260 --140- --100- -70- -50 -40 -35 -25 -15 -10 your gel Log of Molecular Weight 2.415 Estimated Molecular Weight Estimated Molecular Weight
Transcribed Image Text:a travelled by the dye front. DYE FRONT S mm 4. Measure in mm (not cm) the distance travelled (or "migration distance") for each protein in your ladder. Measure from the bottom of the well to each band. 5. Use the Excel Tutorial Video on graphing and solving for the kD sizes of the unknowns. Protein Ladder (for trendline graph): Migration Distance (mm) Rf= Band Migration Dye Front Migration 0-074 0-111 d 17 22, 28. 31 36 Measurements for Unknown A Migration Distance (mm) 4 0-166 0.203 0.314. 0-407 esig 0.574 0,759, ما 14 Measurements for Unknown Migration Distance (mm) 17 Molecular Weight (in KD) 260 250 Rf •074 A Rf 315 JUSLY 20 10 1 kDa --260 --140- --100- -70- -50 -40 -35 -25 -15 -10 your gel Log of Molecular Weight 2.415 Estimated Molecular Weight Estimated Molecular Weight
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