How to prepare Figure 1:. (Refer to Postlab photo of gel for an example.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
How I prepárate this figure ? Please I’m really stuck and I need to understand how to do. It

Transcribed Image Text:esc
How to prepare Figure 1:. (Refer to Postlab photo of gel for an example.
1 of 1
!
1
Q
A. CROP YOUR GEL TO REMOVE EDGES OF PICTURE
B. Add a Label to the top of your gel with lane titles
C. Type the MW of the ladder bands along the side of the gel.
D. (Hint: Click on Gel, Click on "Position" menu, Choose "more layout options". Place photo
behind text. Now you can type along the top and side of the gel easily.)
@
2
W
#3
Caption
E
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4
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Transcribed Image Text:a travelled by the dye front. DYE FRONT S
mm
4. Measure in mm (not cm) the distance travelled (or "migration distance") for each protein in
your ladder. Measure from the bottom of the well to each band.
5. Use the Excel Tutorial Video on graphing and solving for the kD sizes of the unknowns.
Protein Ladder (for trendline graph):
Migration
Distance
(mm)
Rf=
Band Migration
Dye Front Migration
0-074
0-111
d
17
22,
28.
31
36
Measurements for Unknown A
Migration Distance (mm)
4
0-166
0.203
0.314.
0-407
esig
0.574
0,759,
ما
14
Measurements for Unknown
Migration Distance (mm)
17
Molecular Weight
(in KD)
260 250
Rf
•074
A
Rf
315
JUSLY
20
10
1
kDa
--260
--140-
--100-
-70-
-50
-40
-35
-25
-15
-10
your gel
Log of
Molecular Weight
2.415
Estimated Molecular Weight
Estimated Molecular Weight
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