Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Question
You obtained the following raw data when setting up a Biuret standard curve:
| BSA (mg/ml) | Absorbancy 540nm |
| 0 | 0.158 |
| 1 | 0.210 |
| 2 | 0.260 |
| 3 | 0.305 |
| 4 | 0.360 |
| 5 | 0.410 |
| 6 | 0.455 |
| 7 | 0.510 |
| 8 | 0.530 |
| 9 | 0.550 |
| 10 | 0.554 |
After blanking against a biuret-dH2O sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.251 was obtained.
Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / ml (up to 3 significant figures).
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