The nitrate in water was analyzed using a spectrophotometric method. The absorbances of the standards and the unknown sample were obtained at 550 nm. Solution Absorbance 2.00 µM 0.065 6.00 µM 0.205 10.00 µM 0.338 14.00 µM 0.474 18.00 µM 0.598 Unknown 0.402 Write the equation of the line What is the standard deviation of the calibration curve?

Appl Of Ms Excel In Analytical Chemistry

2nd Edition

ISBN:9781285686691

Author:Crouch

Publisher:Crouch

Chapter12: Spectrochemical Methods

Section: Chapter Questions

Problem 12P

See similar textbooks

Related questions

Q: In the process of performing a spectrophotometric determination of Fe, an analyst prepares a…

A: The calibration curve was made by the expert with a certaain cuvette and after it was broken, he…

Q: Atomic absorption analysis of Zn gave a calibration best fit line of A=0.670(ppm Zn) The detection…

A: Given: Standard deviation of the absorbances for 11 samples of the solution =1.560 x 10-3 Slope of…

Q: Using the data below, please create a calibration graph and determine the concentration of the…

Q: Sally obtains a standard calibration curve for their assigned food dye by plotting absorbance versus…

A: The objective of this question is to determine the concentration of the food dye in the original…

Q: Quantitative Analysis by External Standard Method Calcium in a juice sample is determined by atomic…

A: Initial concentration of solution = M1 Volume of starting solution used = V1 Concentration of…

Q: A standard curve was performed using an Absorbance spectrophotometer, and the equation of that line…

Q: To quantify the Cadmium content in a water sample, 5 aliquots of 10 mL were taken, adding to each…

A: The amount of concentration in the sample can be calculated using the following formula. In this…

Q: Copper was determined in a river water sample by atomic absorption spectrometry and the method of…

A: Given,The absorbance of sample, A1 = 0.500The absorbance of sample + blank = 1.020The concentration…

Q: For the determination of Fe3 +, 25 mL of sample taken from sea water is diluted to 100 mL and a…

A: A question based on Beer-Lambert law, which is to be accomplished.

Q: The validation process comprises four main steps that are : hardware validation, software…

A: Validation in analytical chemistry is the process of verifying that a procedure yield acceptable…

Q: 4.00 3.50 3.00 2.50 2.00 1.50 1.00 y = 19883x + 0.5405 R? = 0.8873 0.50 0.00 0.00E+00 5.00E-05…

A: This can be obtained by putting drop line

Q: water sample from the cave was subjected to an analysis to determine ncentration. Using a…

A: We have given that Solution concentration and their Absorbance We have given 5 standard so that by…

Q: 3. The lead in a swab sample, lead standards, together with a blank were made up in 5.00 mL…

A: The amount of lead in the swab sample is approximately 9.44 µg.Explanation:

Q: The equation of your standard curve of absorbance versus concentration (mM) is y = 1.245x + 0.0012.…

A: Beer’s Law- The absorbance of solution is directly proportional to the concentration(c) and path…

Q: 0.1120 g of a solid sample containing copper was dissolved by acid and transferred to a 250.00 mL…

A: Here, by atomic absorption spectrophotometry the amount of copper (Cu) has been measured We know…

Q: Sally obtains a standard calibration curve for their assigned food dye by plotting absorbance versus…

A: Step 1: Relate absorbance to concentration using the calibration curve equationThe calibration curve…

Q: You have prepared several dilutions of a standard compound. You measure the absorbance of each…

Q: The spectroscopic data in the table is generated with five solutions of known concentration.…

A: According to Lambert-Beer's law, the absorbance of the solution is directly proportional to the path…

Q: What is the molar concentration of the unknown sample (in mole L)?

Q: A student prepared four standard solutions of (NH4)2Fe(SO4)2 and measured their absorbance at 505…

A: According to Beer's law absorbance is directly proportional to the concentration Absorbance Vs…

Q: A solution of a dye was analyzed by spectrophotometry, and the following calibration data were…

A: Lambert Beer's law is given as: A=εCl where A is absorbance ε is molar absorptivity coefficient l is…

Q: Atomic absorption spectroscopy was used with the method of standard additions to determine the…

A: To determine the concentration of cadmium in the waste stream sample using the method of standard…

Q: The determination of iron in wines was carried out by atomic absorption spectrophotometry, using the…

A: The conversion relations for the concentration term are shown below.

Q: To test a spectrophotometer’s accuracy, a solution of 60.06 ppm K2Cr2O7 is prepared and analyzed.…

A: For the given solution of potassium chromate with the concentration of 60.06 ppm, the absorbance is…

Q: A calibration curve was created to determine the quantity of protein in a solution. The calibration…

A: Corrected absorbance of any solution is found by deducting the absorbance of the blank solution from…

Q: A calibration curve was created to determine the quantity of protein in a solution. The calibration…

A: Corrected absorbance of any solution is found by deducting the absorbance of the blank solution from…

Q: During your experiment, you measure the absorbance of a sample to be 1.362. You have to convert this…

A: We have to calculate the unknown concentration of the sample.

Q: 10 Wavelength, à. (nm) ɛ (Red, µ.M¯lcm¬ ɛ(Yellow, µ.Mr'cm=l) Absorbance 500 0.0369 0.000549 0.405…

A: The law of Beer-Lambert relates the magnitude of the light to the properties of the medium by which…

Q: A liquid sample was analyzed for Fe³+. A plot of absorbance (y-axis) vs. concentration (ppm; x-axis)…

A: Given that,a liquid sample was analyzed for Fe3+. A plot of absorbance (y-axis) vs. concentration…

Q: From the following data, calculate the concentration of the analyte in the sample read at 700 nm:…

A: As per the Beer–Lambert lawA = a*b*cwhere A = absorbancea = molar absorbtivityb = path lengthc =…

Q: Quinine in a 1.553-g antimalarial tablet was dissolved in sufficient 0.10 M HCl to give 250 mL of…

A: From the Beer Lamberts Law it is clear that absorbance and concentration are directly proportional.…

Q: Using the calibration data above for the dye solution, what is the dye concentration in a solution…

Q: The standard calibration curve for absorbance vs. [Cu"] yielded a straight line with an equation y =…

A: Atomic absorption spectroscopy is used to determine the concentration of unknown samples using a…

Q: After determining the absorbance of several standards of known concentration, the trendline for a…

A: The equation for a straight line is given by y=mx+c where m is the slope and c is the y-intercept.…

Q: An unknown sample of Cu2+ gave an absorbance of 0.262 in an atomic absorption analysis. Then 1.00 mL…

A: As per Lambert Beer's law, absorbance is directly proportional to concentration of the solution.

Concept explainers

UV Visible Spectroscopy

Spectroscopy refers to the absorption or emission of light or other radiations by matter. When a substance or chemical absorbs light or radiation, they reach an excited state followed by de-excitation or emission of the light or radiation forming distinct spectra. UV-visible spectroscopy or ultraviolet-visible refers to the absorption and emission in the ultraviolet and visible regions of the electromagnetic spectrum.

Question

The nitrate in water was analyzed using a spectrophotometric method. The absorbances of the standards and the unknown sample were obtained at 550 nm.

Solution                    Absorbance

2.00 µM                          0.065

6.00 µM                          0.205

10.00 µM                       0.338

14.00 µM                       0.474

18.00 µM                       0.598

Unknown                       0.402

Write the equation of the line
What is the standard deviation of the calibration curve?

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

SEE SOLUTION Check out a sample Q&A here

Step 1

Step 2

Step 3

Step 4

Step 5

Step by step

Solved in 5 steps with 6 images

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.

Similar questions

3. The lead in a swab sample, lead standards, together with a blank were made up in 5.00 mL volumetric flasks using 0.2 % HNO3. 20 μL aliquots of these solutions were injected into the spectrophotometer and the absorbance measured at 217 nm. The following results were obtained. lead / ppb Absorbance 0 0.0591 10.00 0.0858 50.00 0.1926 100.0 0.3260 150.0 0.4594 200.0 0.5929 Swab sample 0.3700 (a) Determine the amount of lead in the swab sample in μg.
Chemistry Atomic absorption analysis of Zn gave a calibration best fit line of A=0.670(ppm Zn) The detection limit was determined for this analysis using a 0.020 ppm Zn solution. The standard deviation of the absorbances for 11 samples of the solution was found to be 1.560 x 10-3 and the slope of the calibration curve was 0.026. Calculate the detection limit, Cq, (in units of ppm) for this instrument. Give your answer to 3 places after the decimal place.
A liquid sample was analyzed for Fe³+. A plot of absorbance (y-axis) vs. concentration (ppm; x-axis) of the external standards produced a calibration line with an equation y = 0.2256x -0.0903 (R² = 0.9973). The absorbance of the sample was 1.25. Determine the amount of Fe³+ in the sample (in ppm).
10 Wavelength, 1. (nm) E (Red, µ.Mcm- ɛ(Yellow, µMr'cm¬1) Absorbance 500 0.0369 0.000549 0.405 430 0.00506 0.0167 0.474 An orange colored solution is prepared by mixing a red dye solution with a yellow dye solution and the absorbance was measured at the analytical wavelengths of 430 nm and 500 nm. The table provides the molar absorptivity for the red and yellow dye at each analytical wavelength. Calculate the concentration (in u.M) of the red and yellow dye in the mixture. [red] = [yellow] =
A sample solution containing quinine was analysed by fluorescence spectroscopy. 5 mL of the sample solution was diluted with 0.1 M HCl to a final volume of 100 mL. The fluorescence of the solution was measured and gave a value of 54. A reference solution containing 0.086 mg/mL of quinine gave a fluorescence reading of 38. A blank solution gave a fluorescence reading of 21. Calculate the quinine content of the sample solution in mg/mL.
A standard curve was performed using an Absorbance spectrophotometer, and the equation of that line was determined to be y = .1563x + .025. The absorbance of an unknown was measured at .35. Calculate the concentration of the unknown according to the standard curve.
0.1040 g of a solid sample containing copper was dissolved by acid and transferred to a 250.00 mL volumetric flask and volume was made up with distilled water. The solution was then diluted by a factor of 20. The final solution was analyzed by atomic absorption spectrophotometry and the concentration was found be 3.109 ppm, What is the weight percentage of copper in the original solid sample? Keep four significant figures in your final answer. Do not try to include the "%" in your answer. e.g. if your final answer is 10.01%, only write 10.01 in the box.
During your experiment, you measure the absorbance of a sample to be 1.362. You have to convert thisabsorbance to methylene blue concentration (in ppm) using the absorbance measurements of two standardsolutions of known concentration:5 ppm: Absorbance 0.55510 ppm: Absorbance 1.211What is, in ppm, the concentration of your sample? Report using 3 decimal figures. Your answer must becorrect to 0.02 ppm
Quinine in a 1.553-g antimalarial tablet was dissolved in sufficient 0.10 M HCl to give 250 mL of solution.A 10.00-mL aliquot was then diluted to 50.00 mL with the acid. The fluorescence intensity for the dilutedsample at 347.5 nm provided a reading of 196 on an arbitrary scale. A standard 100-ppm quinine solutionregistered 125 when measured under conditions identical to those for the diluted sample. Calculate the massin milligrams of quinine in the tablet.
For the determination of Fe3 +, 25 mL of sample taken from sea water is diluted to 100 mL and a quantity of the obtained solution is taken and Fe3 + is determined by atomic absorption spectrophotometer. The absorbance values of the standard and sample solutions are below, and calculate the Fe3 + concentration in sea water in mg / L by drawing the relevant graph.Solution Absorbance1 mg / L Fe3 + 0.04095 mg / L Fe3 + 0.205110 mg / L Fe3 + 0.411615 mg / L Fe3 + 0.620820 mg / L Fe3 + 0.8222Sample Solution 0.5155
0.1120 g of a solid sample containing copper was dissolved by acid and transferred to a 250.00 mL volumetric flask and volume was made up with distilled water. The solution was then diluted by a factor of 25. The final solution was analyzed by atomic absorption spectrophotometry and the concentration was found be 2.982 ppm, What is the weight percentage of copper in the original solid sample? Keep four significant figures in your final answer.
The validation process comprises four main steps that are : hardware validation, software validation, method validation and system suitability. To evaluate a spectrophotometer’s accuracy by measuring absorbance, a solution of 60.06 mg/L K2Cr2O7 in 0.0050M H2SO4 was carefully prepared together with a 0.0050 M H2SO4 as a reagent blank. If the spectrometer’s signal is properly calibrated, it has been reported that this solution should provide an absorbance of 0.640 +- 0.010 at wavelength 350 nm. Which one of the validations mentioned above had been involved in this process? Explain your choice