A calibration curve was created to determine the quantity of protein in a solution. The calibration curve has the form of a straightline with the equationA = 0.0182x + 0.007where A is the corrected absorbance of the solution and x is quantity of protein in the solution in units of micrograms (µg).Determine the quantity of protein in a solution that has an absorbance of 0.344. A blank solution has an absorbance of 0.095.quantity of protein:µg

Answered: A calibration curve was created to…

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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A calibration curve was created to determine the quantity of protein in a solution. The calibration curve has the form of a straight
line with the equation
A = 0.0182x + 0.007
where A is the corrected absorbance of the solution and x is quantity of protein in the solution in units of micrograms (µg).
Determine the quantity of protein in a solution that has an absorbance of 0.344. A blank solution has an absorbance of 0.095.
quantity of protein:
µg

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Corrected absorbance of any solution is found by deducting the absorbance of the blank solution from the absorbance of the solution recorded. This gives the real absorbance value of the solution.

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