A calibration curve was created to determine the quantity of protein in a solution. The calibration curve has the form of a straight line with the equation A = 0.0182x +0.007 where A is the corrected absorbance of the solution and x is quantity of protein in the solution in units of micrograms (µg). Determine the quantity of protein in a solution that has an absorbance of 0.310. A blank solution has an absorbance of 0.053. quantity of protein: µg

A calibration curve was created to determine the quantity of protein in a solution. The calibration curve has the form of a straight line with the equation A = 0.0182x +0.007 where A is the corrected absorbance of the solution and x is quantity of protein in the solution in units of micrograms (µg). Determine the quantity of protein in a solution that has an absorbance of 0.310. A blank solution has an absorbance of 0.053. quantity of protein: µg

Chemistry & Chemical Reactivity

9th Edition

ISBN:9781133949640

Author:John C. Kotz, Paul M. Treichel, John Townsend, David Treichel

Publisher:John C. Kotz, Paul M. Treichel, John Townsend, David Treichel

Chapter4: Stoichiometry: Quantitative Information About Chemical Reactions

Section4.8: Stoichiometry Of Reactions In Aqueous Solution-titrations

Problem 2RC

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UV Visible Spectroscopy

Spectroscopy refers to the absorption or emission of light or other radiations by matter. When a substance or chemical absorbs light or radiation, they reach an excited state followed by de-excitation or emission of the light or radiation forming distinct spectra. UV-visible spectroscopy or ultraviolet-visible refers to the absorption and emission in the ultraviolet and visible regions of the electromagnetic spectrum.

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Macmillan Learning
A calibration curve was created to determine the quantity of protein in a solution. The calibration curve has the form of a
straight line with the equation
A = 0.0182x + 0.007
where A is the corrected absorbance of the solution and x is quantity of protein in the solution in units of micrograms (µg).
Determine the quantity of protein in a solution that has an absorbance of 0.310. A blank solution has an absorbance of 0.053.
quantity of protein:
µg

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