On what basis can one conclude that the enzyme deficiency is associated with the electrontransfer chain and not with the efficiency of proton translocation for ATP synthesis or translocation ofadenine nucleotides across the inner mitochondrial membrane? Where is the enzyme defect located? 3. The following data compare respiratory rates of isolated mitochondria obtained from biopsy tissueof patients with the same enzyme deficiency (uncertainties associated with measurement of respiratoryactivities of patient biopsy tissue are comparable to control values):Substrateaddedpyruvate + malate + ADPglutamate + malate + ADPsuccinate +rotenone + ADPpyruvate + malate + ADPControl(normal)92+ 582 ± 7104 + 111121096+ dinitrophenol (DNP) 95 ± 592(activity units are nanoatoms of oxygen consumed/min/mg protein)Patients2292498963191910393413269995

The electron transfer chain (ETC) is a process which takes place in the mitochondria, the powerhouse…

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On what basis can one conclude that the enzyme deficiency is associated with the electron transfer chain and not with the efficiency of proton translocation for ATP synthesis or translocation of adenine nucleotides across the inner mitochondrial membrane? Where is the enzyme defect located?