The glutamate dehydrogenase (GDH) catalyses the following reaction: *H₂N- H CH₂ CH₂ COO 300° + NAD* + H₂O GDH Time (min) A340 COO CH₂ CH₂ COO O + NH4+ NADH+H* acide glutamique acide a-cétoglutarique The activity of GDH is monitored in the sense of the formation of glutamate using the following conditions: -0.2 mL of 5 M ammonium sulphate - 2.4 mL of buffer at pH 8 - 0.1 mL of NADH at 6.15 mg.mL-¹ (M = 709 g.mol-¹) - 0.2 mL of 1 M a-ketoglutarate solution Warm mixture at 25 °C for 5 min Add 0.1 mL of GDH solution containing 1.6 mg.mL 'protein to start the reaction. The change in absorbance at 340 nm is monitored, in a 1-cm cuvette, every minute for 10 min. Results are given in the table below: Data: ENADH at 340 nm = 6220 M¹.cm¹ 4 5 6 7 8 1 2 3 9 1.760 1.718 1.675 1.635 1.595 1.550 1.510 1.489 1.476 - Draw the graph A = f(t). Calculate A340 at t = 0 and place this point on the curve. - Comment the shape of the curve, particularly the portion that corresponds to a plateau. 10 1.451
The glutamate dehydrogenase (GDH) catalyses the following reaction: *H₂N- H CH₂ CH₂ COO 300° + NAD* + H₂O GDH Time (min) A340 COO CH₂ CH₂ COO O + NH4+ NADH+H* acide glutamique acide a-cétoglutarique The activity of GDH is monitored in the sense of the formation of glutamate using the following conditions: -0.2 mL of 5 M ammonium sulphate - 2.4 mL of buffer at pH 8 - 0.1 mL of NADH at 6.15 mg.mL-¹ (M = 709 g.mol-¹) - 0.2 mL of 1 M a-ketoglutarate solution Warm mixture at 25 °C for 5 min Add 0.1 mL of GDH solution containing 1.6 mg.mL 'protein to start the reaction. The change in absorbance at 340 nm is monitored, in a 1-cm cuvette, every minute for 10 min. Results are given in the table below: Data: ENADH at 340 nm = 6220 M¹.cm¹ 4 5 6 7 8 1 2 3 9 1.760 1.718 1.675 1.635 1.595 1.550 1.510 1.489 1.476 - Draw the graph A = f(t). Calculate A340 at t = 0 and place this point on the curve. - Comment the shape of the curve, particularly the portion that corresponds to a plateau. 10 1.451
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
![The glutamate dehydrogenase (GDH) catalyses the following reaction:
+H₂N-
H
-
-
CH₂
-
CH₂
COO
acide glutamique
COO™®
+ NAD+ + H₂O
GDH
COO
C:
CH₂
CH₂
COO™
O
+ NH4+ NADH + H*
The activity of GDH is monitored in the sense of the formation of glutamate using the
following conditions:
-0.2 mL of 5 M ammonium sulphate
2.4 mL of buffer at pH 8
0.1 mL of NADH at 6.15 mg.mL-¹ (M = 709 g.mol-¹)
0.2 mL of 1 M a-ketoglutarate solution
Warm mixture at 25 °C for 5 min
- Add 0.1 mL of GDH solution containing 1.6 mg.mL-¹protein to start the
reaction.
acide a-cétoglutarique
The change in absorbance at 340 nm is monitored, in a 1-cm cuvette, every minute
for 10 min. Results are given in the table below:
Data: ENADH at 340 nm = 6220 M¹.cm¹
Time (min) 1 2 3 4 5 6 7 8 9
1.760 1.718 1.675 1.635 1.595 1.550 1.510 1.489 1.476
A340
10
1.451
- Draw the graph A = f(t). Calculate A340 at t = 0 and place this point on the curve.
- Comment the shape of the curve, particularly the portion that corresponds to a
plateau.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Ff1cc82da-1a95-413d-800c-76a0d9bb28c9%2F1301ba9d-a3ce-4b01-be53-8ee4f1bac8bf%2F8ysgzxq_processed.jpeg&w=3840&q=75)
Transcribed Image Text:The glutamate dehydrogenase (GDH) catalyses the following reaction:
+H₂N-
H
-
-
CH₂
-
CH₂
COO
acide glutamique
COO™®
+ NAD+ + H₂O
GDH
COO
C:
CH₂
CH₂
COO™
O
+ NH4+ NADH + H*
The activity of GDH is monitored in the sense of the formation of glutamate using the
following conditions:
-0.2 mL of 5 M ammonium sulphate
2.4 mL of buffer at pH 8
0.1 mL of NADH at 6.15 mg.mL-¹ (M = 709 g.mol-¹)
0.2 mL of 1 M a-ketoglutarate solution
Warm mixture at 25 °C for 5 min
- Add 0.1 mL of GDH solution containing 1.6 mg.mL-¹protein to start the
reaction.
acide a-cétoglutarique
The change in absorbance at 340 nm is monitored, in a 1-cm cuvette, every minute
for 10 min. Results are given in the table below:
Data: ENADH at 340 nm = 6220 M¹.cm¹
Time (min) 1 2 3 4 5 6 7 8 9
1.760 1.718 1.675 1.635 1.595 1.550 1.510 1.489 1.476
A340
10
1.451
- Draw the graph A = f(t). Calculate A340 at t = 0 and place this point on the curve.
- Comment the shape of the curve, particularly the portion that corresponds to a
plateau.
Expert Solution
![](/static/compass_v2/shared-icons/check-mark.png)
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
Step by step
Solved in 2 steps with 1 images
![Blurred answer](/static/compass_v2/solution-images/blurred-answer.jpg)
Recommended textbooks for you
![Biochemistry](https://www.bartleby.com/isbn_cover_images/9781319114671/9781319114671_smallCoverImage.jpg)
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
![Lehninger Principles of Biochemistry](https://www.bartleby.com/isbn_cover_images/9781464126116/9781464126116_smallCoverImage.gif)
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
![Fundamentals of Biochemistry: Life at the Molecul…](https://www.bartleby.com/isbn_cover_images/9781118918401/9781118918401_smallCoverImage.gif)
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
![Biochemistry](https://www.bartleby.com/isbn_cover_images/9781319114671/9781319114671_smallCoverImage.jpg)
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
![Lehninger Principles of Biochemistry](https://www.bartleby.com/isbn_cover_images/9781464126116/9781464126116_smallCoverImage.gif)
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
![Fundamentals of Biochemistry: Life at the Molecul…](https://www.bartleby.com/isbn_cover_images/9781118918401/9781118918401_smallCoverImage.gif)
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
![Biochemistry](https://www.bartleby.com/isbn_cover_images/9781305961135/9781305961135_smallCoverImage.gif)
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
![Biochemistry](https://www.bartleby.com/isbn_cover_images/9781305577206/9781305577206_smallCoverImage.gif)
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
![Fundamentals of General, Organic, and Biological …](https://www.bartleby.com/isbn_cover_images/9780134015187/9780134015187_smallCoverImage.gif)
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON