The catalytic mechanism of an enzyme found in the mitochondrial matrix (pH = 7.8) depends on an active site Lys residue (pKa = 9.5) that acts as a general acid. Mutation of a neighboring residue alters the microenvironment of the Lys side chain so that its pKa shifts from 9.5 to 7.2. Would the mutation cause the reaction rate to increase or decrease? Explain your answer.

Biochemistry
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Chapter1: Biochemistry: An Evolving Science
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The catalytic mechanism of an enzyme found in the mitochondrial matrix (pH = 7.8) depends on an active site Lys residue (pKa = 9.5) that acts as a general acid. Mutation of a neighboring residue alters the microenvironment of the Lys side chain so that its pKa shifts from 9.5 to 7.2. Would the mutation cause the reaction rate to increase or decrease? Explain your answer.

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