based on the chart answer the following question1. which protein will have the most amount of attraction to an anion column operating at the physiological ph ProteinAldolaseCytochrome CLactate dehydrogenaseOrnithine decarboxylasePEP carboxylasePyruvate kinaseMolecular weight (Da)40,00014,00018,00075,00074,00062,000pl10.19.28.54.15.06.0

pH (Potential of Hydrogen):It is a measure of the acidity or alkalinity of a solution.It quantifies…

Answered: most amount of attraction to an anion…

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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based on the chart answer the following question

1. which protein will have the most amount of attraction to an anion column operating at the physiological ph

Expert Solution

Step 1: Explain pH and pI

pH (Potential of Hydrogen):

It is a measure of the acidity or alkalinity of a solution.
It quantifies the concentration of H+ ions in a solution.
The scale ranges from 0 to 14, with 7 being considered neutral.
When the pH is less than 7 it indicates acidicty, with lower values representing stronger acidity.
When the pH is greater than 7 it indicates alkalinity, with higher values representing stronger alkalinity.

pI (isoelectric point):

It is the characteristic feature of a protein or amino acid
It is the pH at which the molecule is electrically neutral.
This is because the number of positively charged protons (H+) and negatively charged hydroxide ions (OH-) are equal.
It also means that a molecule has an equal number of positively and negatively charged groups.
The molecule will have a net negative charge above the pI, and a net positive charge below the pI.
It is an important parameter in various biochemical and biophysical processes such as electrophoresis and chromatography.