Three cell membrane samples: M, C, and S were fluorescently labeled and were subsequently photobleached. The recovery of fluorescence was monitored and gave the following profile: 100 M S time a. Rank the three cell membranes in terms of decreasing fluidity. Provide an explanation of your ranking based on their FRAP curves and the most probable appearance and composition of the three membranes. b. Draw the expected image of the surface of the three membranes before photobleaching and after fluorescence recovery as viewed under a fluorescence microscope. % fluorescence

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Answer the question a and b. Use image b as guide
Hydropathy Plots
10
50
100
150
200
250
3
↑
Hydrophobic
Hydrophilic
Amino
terminus
Outside
-3
10
50
100
150
200
250
Residue number
Bacteriorhodopsin
Inside
Carboxyl
terminus
Hydropathy index
Transcribed Image Text:Hydropathy Plots 10 50 100 150 200 250 3 ↑ Hydrophobic Hydrophilic Amino terminus Outside -3 10 50 100 150 200 250 Residue number Bacteriorhodopsin Inside Carboxyl terminus Hydropathy index
Three cell membrane samples: M, C, and S were fluorescently labeled and were subsequently
photobleached. The recovery of fluorescence was monitored and gave the following profile:
100
M
S
time
a. Rank the three cell membranes in terms of decreasing fluidity. Provide an explanation of
your ranking based on their FRAP curves and the most probable appearance and composition
of the three membranes.
b. Draw the expected image of the surface of the three membranes before photobleaching
and after fluorescence recovery as viewed under a fluorescence microscope.
% fluorescence
Transcribed Image Text:Three cell membrane samples: M, C, and S were fluorescently labeled and were subsequently photobleached. The recovery of fluorescence was monitored and gave the following profile: 100 M S time a. Rank the three cell membranes in terms of decreasing fluidity. Provide an explanation of your ranking based on their FRAP curves and the most probable appearance and composition of the three membranes. b. Draw the expected image of the surface of the three membranes before photobleaching and after fluorescence recovery as viewed under a fluorescence microscope. % fluorescence
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