Mingyu isolated double-stranded (ds) DNA from Matricaria chamomilla, a medicinal plant that is used as a sedative, antispasmodic, antiseptic, and antiemetic. From the ds-DNA isolate, Mingyu obtained 2.50 mL and diluted it with 12.00 mL of distilled water. He then subjected the diluted ds-DNA isolate to spectrophotometric analysis. Unfortunately, Mingyu lost the rest of his data except for the actual concentration of the ds-DNA isolate, which is equal to 149.70 µg/mL. Help Mingyu determine the following: For items 1-3 and 7, report your answers in 3 decimal places while for items 4-6, report your answers in the nearest whole numbers. 1. diluted concentration (in µg/mL) of the ds-DNA isolate 2. absorbance at 260 nm of the ds-DNA isolate 3. absorbance at 280 nm assuming that the ds-DNA isolate is pure
Mingyu isolated double-stranded (ds) DNA from Matricaria chamomilla, a medicinal plant that is used as a sedative, antispasmodic, antiseptic, and antiemetic. From the ds-DNA isolate, Mingyu obtained 2.50 mL and diluted it with 12.00 mL of distilled water. He then subjected the diluted ds-DNA isolate to spectrophotometric analysis. Unfortunately, Mingyu lost the rest of his data except for the actual concentration of the ds-DNA isolate, which is equal to 149.70 µg/mL. Help Mingyu determine the following: For items 1-3 and 7, report your answers in 3 decimal places while for items 4-6, report your answers in the nearest whole numbers. 1. diluted concentration (in µg/mL) of the ds-DNA isolate 2. absorbance at 260 nm of the ds-DNA isolate 3. absorbance at 280 nm assuming that the ds-DNA isolate is pure
Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
Publisher:ESTRIDGE
Chapter2: Basic Hematology
Section2.3: Microhematocrit
Problem 3RQ
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Transcribed Image Text:Mingyu isolated double-stranded (ds) DNA from Matricaria chamomilla, a medicinal plant that is used
as a sedative, antispasmodic, antiseptic, and antiemetic. From the ds-DNA isolate, Mingyu obtained 2.50
mL and diluted it with 12.00 mL of distilled water. He then subjected the diluted ds-DNA isolate to
spectrophotometric analysis. Unfortunately, Mingyu lost the rest of his data except for the actual
concentration of the ds-DNA isolate, which is equal to 149.70 µg/mL. Help Mingyu determine the
following: For items 1-3 and 7, report your answers in 3 decimal places while for items 4-6, report
your answers in the nearest whole numbers.
1. diluted concentration (in µg/mL) of the ds-DNA isolate
2. absorbance at 260 nm of the ds-DNA isolate
3. absorbance at 280 nm assuming that the ds-DNA isolate is pure
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