2 of 3You want to study the oxygen binding ability of hemoglobin at pH 7.4. How wouldyou make up 500 ml of 75 mM phosphate buffer, pH 7.4, starting with 1.00 M Na3PO4 and either2 M HCl or 3 M NaOH? The pKa's of phosphoric acid are 2.12, 7.21, and 12.32. Show all calculations.

To make 500 ml of 75 mM phosphate buffer, pH 7.4, starting with 1.00 M Na3PO4, you can use the…

Answered: You want to study the oxygen binding…

Human Physiology: From Cells to Systems (MindTap Course List)

9th Edition

ISBN:9781285866932

Author:Lauralee Sherwood

Publisher:Lauralee Sherwood

Chapter11: The Blood

Section: Chapter Questions

Problem 1SQE

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Which of the following is correct concerning the oxygenation plot of proteins X and Y shown below? Y (fraction saturation) 1.0 0.8 0.6 등 0.4 0.2 0.0 0 20 Oxygen binding plot 40 flog ha 60 p02 (torr) 80 100 X Y
at 257 nm) is dissolved in 2.5 mL of buffer. A 250 microliter aliquot is removed and placed in a 1.0 cm A sample of adenosine triphosphate (ATP) (MW 507, ɛ = 14,700 M'cm cuvette with sufficient buffer to give a total volume of 2.0 mL. The absorbance of the sample at 257 nm is 2.0. Calculate the weight of ATP in the original 2.5 mL sample. (Choose the correct value from the drop-down list provided. The values of weight are listed in units of milligrams.) Weight of ATP: v mg
Highly active muscles generate lactic acid by respiration so fast that the blood bassing through the muscle actually experiences a drop in pH from 7.4 to 7.2. Under these conditions, hemoglobin releases about 10% more O2 than it does at pH 7.4. Explain.
The figure shows three oxygen binding curves for hemoglobin in the red blood cells of normal blood, pH 7.6. The middle-dashed line is the standard line for human hemoglobin. Lines A and B represent two experimental conditions your lab partner plotted but forgot to label. 1 0.8 0.6 0.4 0.2 | pO2 Q. which one is the hemoglobin you reduced the concentration of 2,3- bisphosphoglycerate in? and which line is the data from the hemoglobin you changed the pH to 7.3?
If we were handed a tuke of 2mg/mL BSA how much is required 20μL of each of the following Concentrations? to make zoul total bring up to volume with dH ₂0 @ 01125 mg/mL (1) 0,150 mg Ⓒ 0.50 mg/mL @ 0.75 mg/mL Ⓒ 1.0mg/ml /ML Can it be solved using C₂ C₁V₁ = C₂ V₂ If CI= V₁ yes, please show work. Vz
3. (a) The (acid) Bohr effect is defined as the up- take of protons by hemoglobin molecules at pH ≤ 6.5 as O2 is dissociated from the protein in solution. Approxi- mately 0.6 mole protons are taken up for each mole of O2 released by normal human hemoglobin. The effect is seen as a "shift to the right" in the O2 dissociation curves with decrease in pH, indicating that the O2 binding affinity of Hb decreases at lower pH. On the right is a diagram compar- ing the pH dependence of the p50 values of Hb Deer Lodge [His(32)Arg] (filled symbols) and of HbA (- - -) in the absence of organic phosphates, i.e., “Stripped”, and in the presence of saturating concentrations of IHP. On the basis of the definition of the Bohr effect given above, explain why the data in the diagram reflect the Bohr effect for the two types of human hemoglobins. log p50 1.5 1.0 0.5 0.0 -0.5 5 6 stripped 7 pH +DPG 8 IHP 9 (b) The Bohr effect is generally decreased in Hb Deer Louge compared to that in HbA. Provide an…
Acetic acid is the principal ingredient in vinegar as shown; that's why it tastes sour. At equilibrium, a solution contains [CH3CO2H] = 0.0787 M and [H3 O+] = [CH3 CO2−] = 0.00118 M. What is the value of Ka for acetic acid?
Give detailed Solution with explanation (no need Handwritten
1. Draw an approximate titration curve for lysine, given that its pKa(COOH) = 2.18, its pKa(NH3*) = 8.95, and its pka(R) = 10.53. a. What pH ranges would Lys be a good buffer at? b. What is the pl of Lys? C. Draw the structures of the predominant species in solution at (i) pH 0, (ii) pH 7, (iii) pH 9.5, (iv) pH 12.
None
7l.4.3
A patient presents to the emergency department in a critical condition. The patient’s blood was tested and the concentration of carbonic acid (H2CO3) was found to be 4.0×10-4 M and bicarbonate ion (HCO3-) 3.062×10-3 M. Ka = 4.3 × 10-7 H2CO3(aq) ⇌ HCO3-(aq) + H+(aq) Show all calculations including equation(s) used. Calculate the pH of the patients’ blood. Based on your answer would you say the patient is suffering from Acidosis or Alkalosis?

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You want to study the oxygen binding ability of hemoglobin at pH 7.4. How would you make up 500 ml of 75 mM phosphate buffer, pH 7.4, starting with 1.00 M Na3PO4 and either 2 M HCl or 3 M NaOH? The pKa's of phosphoric acid are 2.12, 7.21, and 12.32. Show all calculations.