2 of 3You want to study the oxygen binding ability of hemoglobin at pH 7.4. How wouldyou make up 500 ml of 75 mM phosphate buffer, pH 7.4, starting with 1.00 M Na3PO4 and either2 M HCl or 3 M NaOH? The pKa's of phosphoric acid are 2.12, 7.21, and 12.32. Show all calculations.

To make 500 ml of 75 mM phosphate buffer, pH 7.4, starting with 1.00 M Na3PO4, you can use the…

Answered: You want to study the oxygen binding…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

See similar textbooks

Similar questions

( Please type answer note write by hend )
Please draw a visual representation
You have been observing an insect that defends itself from enemies by secreting a caustic liquid. Analysis of the liquid shows it to have a total concentration of formate plus formic acid (K₁ = 1.8 x 10-4) of 1.45 M. Further analysis reveals that the concentration of formate ion is 0.015 M. What is the pH of the secretion? pH =
The following represents the saturation curve of 2 proteins that bind oxygen. The red line represents the saturation curve for protein A while the blue line represents protein B. 100 80 60 40 20 10 20 30 40 50 60 70 pO2 (mmHg) Which of the following statements about the figure is correct? A. Protein A is a monomer, while protein B is a tetramer B. Protein A is myoglobin while protein B is hemoglobin C. Protein A shows noncooperative binding while protein B exhibits cooperative binding for oxygen. D. Protein A has a higher affinity and therefore binds faster with oxygen while protein B has a lower affinity for binding to oxygen. E. Protein A will bind more oxygen and therefore has a higher saturation than protein B. (%] ?0}S
Why HCL is more concentrated than NaOH in titration?(pls explain thoroughly and cite some example)
The side chain of acetyl-L-lysine-OMe has a pKa=10.5 and its acid-dissociation reaction is shown below. see attachment for Question D.
What is respiratory quotient? Calculate the respiratory quotient for each of the following substances. (a) Malic acid (b) Oleic acid (c) Palmitic acid. (d) Triolein (e) Acetic acid
Chemistry CO(g) + 3H2(g) ⇌ CH4(g) + H2O(g) Happens @ 300degrees Celsius The equilibrium concentrations of CO, H2, CH4 and H2O are 12.6 Pa, 7.6 Pa 6.06 Pa and 15.5 Pa respectively. The Kp value is 0.017. a) Determine the value of Kc at 300 °C (Gas constant = 8.315 Pa m3 K-1 mol-1) and calculate the Kp value @ 800 degrees Celsius if the equilibrium concentration of CH4 @ 800 degrees Celsius is 4.6 Pa b) What is the forward reaction exothermic or endothermic? Give a reason
A researcher wants to look for glucaronic acid conjugated metabolites in sample of urine(glucaronic acid conjugation is a common method of metabolism for excretion.) Pronotated glucaronic acid conjugated metabolite ions often lose anhydroglucaronic acid(176 Da) during low energy CID. Which triple quadrupole experiment might be best for finding these conjugates?
Protein Solubility 3.5 5.5 5 5 0.068 0.028 Absorbance 0.098 Conc.(mg/ml) 0.195 0.130 0.044 0.9% 2.786% 9.429% 11.3: % solubility 4.179% The above table indicates the concentration of protein in the diluted supernatant and the supernatant before dilution at different pH. Dilution Factor 30 22.5 15 PH 7.5 1.5 4.5 5 0.027 3 0.042 Protein Solubility Versus pH 6.5 6 4.5 pH Value The above figure shows a plot of protein solubility versus pH. 50 7.5 7.5 50 0.032 0.053 8.5 50 0.054 0.100 21.429% 9 Please provide a brief discussion and explanation of the results. (using isoelectric point and net charge to explain)
The figure shows three oxygen binding curves for hemoglobin in the red blood cells of normal blood, pH 7.6. The middle-dashed line is the standard line for human hemoglobin. Lines A and B represent two experimental conditions your lab partner plotted but forgot to label. 1 0.8 0.6 0.4 0.2 | pO2 Q. which one is the hemoglobin you reduced the concentration of 2,3- bisphosphoglycerate in? and which line is the data from the hemoglobin you changed the pH to 7.3?
NaOH.2L X.02mol/L = .004 mol, H3PO4.8L X.15 mol/L = .12 mol H₂PO4 HPO4² + H+ .108 mol start A. If 200 mls of 0.02 M NaoH is added to 800 mls of 0.15 M phosphoric acid buffer at pH 8.2, what is the resultant pH? (pK1 = 2.1, Pk2=7.2, pk3=12.2) add .004 mol OH- end .012mol -.004 .008 mol pH= pka + log [A-]/[HA] = 7.2 + log.112/.008 +.004 .112mol pH=8.35 B. If the same amount of NaOH in part A is added to 800 mls of water, what is the resulting pH? .004mol/1L =.004 [OH-] pOH = 2.4, pH -11.6

SEE MORE QUESTIONS

Recommended textbooks for you

Biochemistry

ISBN:

9781319114671

Author:

Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:

W. H. Freeman

Lehninger Principles of Biochemistry

Biochemistry

ISBN:

9781464126116

Author:

David L. Nelson, Michael M. Cox

Publisher:

W. H. Freeman

Fundamentals of Biochemistry: Life at the Molecul…

Biochemistry

ISBN:

9781118918401

Author:

Donald Voet, Judith G. Voet, Charlotte W. Pratt

Publisher:

WILEY

Biochemistry

ISBN:

9781319114671

Author:

Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:

W. H. Freeman

Lehninger Principles of Biochemistry

Biochemistry

ISBN:

9781464126116

Author:

David L. Nelson, Michael M. Cox

Publisher:

W. H. Freeman

Fundamentals of Biochemistry: Life at the Molecul…

Biochemistry

ISBN:

9781118918401

Author:

Donald Voet, Judith G. Voet, Charlotte W. Pratt

Publisher:

WILEY

Biochemistry

ISBN:

9781305961135

Author:

Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal

Publisher:

Cengage Learning

Biochemistry

ISBN:

9781305577206

Author:

Reginald H. Garrett, Charles M. Grisham

Publisher:

Cengage Learning

Fundamentals of General, Organic, and Biological …

Biochemistry

ISBN:

9780134015187

Author:

John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson

Publisher:

PEARSON

SEE MORE TEXTBOOKS

You want to study the oxygen binding ability of hemoglobin at pH 7.4. How would you make up 500 ml of 75 mM phosphate buffer, pH 7.4, starting with 1.00 M Na3PO4 and either 2 M HCl or 3 M NaOH? The pKa's of phosphoric acid are 2.12, 7.21, and 12.32. Show all calculations.