2 of 3You want to study the oxygen binding ability of hemoglobin at pH 7.4. How wouldyou make up 500 ml of 75 mM phosphate buffer, pH 7.4, starting with 1.00 M Na3PO4 and either2 M HCl or 3 M NaOH? The pKa's of phosphoric acid are 2.12, 7.21, and 12.32. Show all calculations.

To make 500 ml of 75 mM phosphate buffer, pH 7.4, starting with 1.00 M Na3PO4, you can use the…

Answered: You want to study the oxygen binding…

Human Physiology: From Cells to Systems (MindTap Course List)

9th Edition

ISBN:9781285866932

Author:Lauralee Sherwood

Publisher:Lauralee Sherwood

Chapter11: The Blood

Section: Chapter Questions

Problem 1SQE

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Similar questions

At a pH of 7.40, the carbonic acid ratio is ________. a. 35:1 b. 4:1 c. 20:1 d. 3:1
12. Metabolic acidosis caused by fixed acids is present when the anion gap is greater than A. 9 mEq/L B. 14 mEq/L C. 20 mEq/L D. 25 mEq/L
Plasma contains more sodium than chloride. How can this be if individual ions of sodium and chloride exactly balance each other out, and plasma is electrically neutral?
Ty is three years old and as a result of a stomach bug has been vomiting for about 24 hours. His blood pH is 7.48. What does this mean? Tys blood is slightly acidi Tys blood is slightly alkaline. Tys blood is highly acidi Tys blood is within the normal range
MATHEMATICAL A catalog in the lab has a recipe for preparing 1 L of a TRIS buffer at 0.0500 M and with pH 8.0: dissolve 2.02 g of TRIS (free base, MW=121.1 g/mol) and 5.25 g of TRIS hydrochloride (the acidic form, MW=157.6 g/mol) in a total volume of 1 L. Verify that this recipe is correct.
In each of the three systems, determine the state of entropy (low or high) when comparing the first and second: i. the instant that a perfume bottle is sprayed compared with 30 seconds later, ii. an old 1950s car compared with a brand new car, and iii. a living cell compared with a dead cell. a. i. low, ii. high, iii. low b. i. low, ii. high, iii. high c. i. high, ii. low, iii. high d. i. high, ii. low, iii. low

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You want to study the oxygen binding ability of hemoglobin at pH 7.4. How would you make up 500 ml of 75 mM phosphate buffer, pH 7.4, starting with 1.00 M Na3PO4 and either 2 M HCl or 3 M NaOH? The pKa's of phosphoric acid are 2.12, 7.21, and 12.32. Show all calculations.