Louie performed paper chromatography to identify which among the four available reference mixtures WAR, OFF, YIN, MEW – have the same components as his unknown sample, GUN. He spotted the standards and the sample in a Whatman No. 1 filter paper, which he placed in a developing chamber containing 3% KCl. After 20 minutes, Louie obtained the chromatogram below, immediately noting that the distance travelled by the solvent from the origin is 79.0 mm. On the other hand, the distances (in cm) traveled by the developed spots were tabulated as follows. solvent front- H I A C origin WAR OFF ΥIN MEW GUN DISTANCE DISTANCE TRAVELLED FROM SPOTS TRAVELLED FROM SPOTS THE ORIGIN, cm THE ORIGIN, cm A 2.70 F 5.40 4.75 G 2.90 2.25 H 4.10 2.85 3.50 I 3.25 4.95 1. Is the type of paper chromatography performed normal or reversed? Why?

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Louie performed paper chromatography to identify which among the four available
reference mixtures
WAR, OFF, YIN, MEW – have the same components as his
unknown sample, GUN. He spotted the standards and the sample in a Whatman No. 1
filter paper, which he placed in a developing chamber containing 3% KCl. After 20
minutes, Louie obtained the chromatogram below, immediately noting that the distance
travelled by the solvent from the origin is 79.0 mm. On the other hand, the distances (in
cm) traveled by the developed spots were tabulated as follows.
solvent front-
H
I
A
C
origin
WAR
OFF
ΥIN
MEW
GUN
DISTANCE
DISTANCE TRAVELLED FROM
SPOTS
TRAVELLED FROM
SPOTS
THE ORIGIN, cm
THE ORIGIN, cm
A
2.70
F
5.40
4.75
G
2.90
2.25
H
4.10
2.85
3.50
I
3.25
4.95
1. Is the type of paper chromatography performed normal or reversed? Why?
Transcribed Image Text:Louie performed paper chromatography to identify which among the four available reference mixtures WAR, OFF, YIN, MEW – have the same components as his unknown sample, GUN. He spotted the standards and the sample in a Whatman No. 1 filter paper, which he placed in a developing chamber containing 3% KCl. After 20 minutes, Louie obtained the chromatogram below, immediately noting that the distance travelled by the solvent from the origin is 79.0 mm. On the other hand, the distances (in cm) traveled by the developed spots were tabulated as follows. solvent front- H I A C origin WAR OFF ΥIN MEW GUN DISTANCE DISTANCE TRAVELLED FROM SPOTS TRAVELLED FROM SPOTS THE ORIGIN, cm THE ORIGIN, cm A 2.70 F 5.40 4.75 G 2.90 2.25 H 4.10 2.85 3.50 I 3.25 4.95 1. Is the type of paper chromatography performed normal or reversed? Why?
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