Louie performed paper chromatography to identify which among the four available reference mixtures WAR, OFF, YIN, MEW – have the same components as his unknown sample, GUN. He spotted the standards and the sample in a Whatman No. 1 filter paper, which he placed in a developing chamber containing 3% KCl. After 20 minutes, Louie obtained the chromatogram below, immediately noting that the distance travelled by the solvent from the origin is 79.0 mm. On the other hand, the distances (in cm) traveled by the developed spots were tabulated as follows. solvent front- H I A C origin WAR OFF ΥIN MEW GUN DISTANCE DISTANCE TRAVELLED FROM SPOTS TRAVELLED FROM SPOTS THE ORIGIN, cm THE ORIGIN, cm A 2.70 F 5.40 4.75 G 2.90 2.25 H 4.10 2.85 3.50 I 3.25 4.95 1. Is the type of paper chromatography performed normal or reversed? Why?

Louie performed paper chromatography to identify which among the four available reference mixtures WAR, OFF, YIN, MEW – have the same components as his unknown sample, GUN. He spotted the standards and the sample in a Whatman No. 1 filter paper, which he placed in a developing chamber containing 3% KCl. After 20 minutes, Louie obtained the chromatogram below, immediately noting that the distance travelled by the solvent from the origin is 79.0 mm. On the other hand, the distances (in cm) traveled by the developed spots were tabulated as follows. solvent front- H I A C origin WAR OFF ΥIN MEW GUN DISTANCE DISTANCE TRAVELLED FROM SPOTS TRAVELLED FROM SPOTS THE ORIGIN, cm THE ORIGIN, cm A 2.70 F 5.40 4.75 G 2.90 2.25 H 4.10 2.85 3.50 I 3.25 4.95 1. Is the type of paper chromatography performed normal or reversed? Why?

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Louie performed paper chromatography to identify which among the four availablereference mixtures — WAR, OFF, YIN, MEW — have the same components as hisunknown sample, GUN. He spotted the standards and the sample in a Whatman No. 1filter paper, which he placed in a developing chamber containing 3% KCl. After 20minutes, Louie obtained the chromatogram below, immediately noting that the distancetravelled by the solvent from the origin is 79.0 mm. On the other hand, the distances (incm) traveled by the developed spots were tabulated as follows.(see attached) Questions: 1. Is the type of paper chromatography performed normal or reversed? Why?2. Calculate the Rf of the spots from the samples. Report your answers in four decimal places.
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