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b) In the separation of a sample containing five polar compounds, chromatogram A was obtained using an isocratic mixture of hexane:acetone with a ratio of 75:25. The separation was improved (chromatogram B) using a gradient elution approach with the same mobile phase mixture. Suggest the gradient elution program used in obtaining chromatogram B. Explain your answer. mAU 2 40 3 5 30 20 10 -10 1 2 3 4 6 min Chromatogram A mAU 40 3 30 5 20 10 -10 2 4. 6 min Chromatogram B

b) In the separation of a sample containing five polar compounds, chromatogram A was obtained using an isocratic mixture of hexane:acetone with a ratio of 75:25. The separation was improved (chromatogram B) using a gradient elution approach with the same mobile phase mixture. Suggest the gradient elution program used in obtaining chromatogram B. Explain your answer. mAU 2 40 3 5 30 20 10 -10 1 2 3 4 6 min Chromatogram A mAU 40 3 30 5 20 10 -10 2 4. 6 min Chromatogram B

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
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Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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b) In the separation of a sample containing five polar compounds, chromatogram A was obtained using an isocratic mixture of hexane:acetone with a ratio of 75:25. The separation was improved (chromatogram B) using a gradient elution approach with the same mobile phase mixture. Suggest the gradient elution program used in obtaining chromatogram B. Explain your answer. mAU 2 40 3 5 30 20 10 -10 1 2 3 4 6 min Chromatogram A mAU 40 3 30 5 20 10 -10 4. 6 min Chromatogram B
Transcribed Image Text:b) In the separation of a sample containing five polar compounds, chromatogram A was obtained using an isocratic mixture of hexane:acetone with a ratio of 75:25. The separation was improved (chromatogram B) using a gradient elution approach with the same mobile phase mixture. Suggest the gradient elution program used in obtaining chromatogram B. Explain your answer. mAU 2 40 3 5 30 20 10 -10 1 2 3 4 6 min Chromatogram A mAU 40 3 30 5 20 10 -10 4. 6 min Chromatogram B
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