Louie performed paper chromatography to identify which among the four available reference mixtures — WAR, OFF, YIN, MEW — have the same components as his unknown sample, GUN. He spotted the standards and the sample in a Whatman No. 1 filter paper, which he placed in a developing chamber containing 3% KCl. After 20 minutes, Louie obtained the chromatogram below, immediately noting that the distance
Louie performed paper chromatography to identify which among the four available
reference mixtures — WAR, OFF, YIN, MEW — have the same components as his
unknown sample, GUN. He spotted the standards and the sample in a Whatman No. 1
filter paper, which he placed in a developing chamber containing 3% KCl. After 20
minutes, Louie obtained the chromatogram below, immediately noting that the distance
travelled by the solvent from the origin is 79.0 mm. On the other hand, the distances (in
cm) traveled by the developed spots were tabulated as follows.(see attached)
Questions:
1. Is the type of paper chromatography performed normal or reversed? Why?
2. Calculate the Rf of the spots from the samples. Report your answers in four decimal places.
Step by step
Solved in 5 steps with 1 images
