12. A standard solution with known concentrations of tartaric acid and fumaric acid was prepared and measured using reversed phase liquld chromatography. The data for known concentrations is shown in the table below: Concentration (ppm) Peak Area Sample mobile phase N/A N/A tartaric acid 455 369 fumaric acid 125 249 A second solution containing 5.00 mL of an unknown concentration of tartaric acid was combined with 10.0 ml of 81.0 ppm of fumaric acid and diluted with water to 100.00 mL in a 100.00 ml volumetric flask. The chromatogram of this mixture produced peak areas of 244 and 125 for tartaric acid and fumaric acid, respectively. a. What type of calibration method is used in this analysis and how do you know? b. In this analysis, the stationary phase is: Circle Oner: POLAR NO POLAR C. By increasing the eluent strength of the mobile phase (making it more like the column polarity), how would the retention time of the analyte change in the analysis? Circle Ones INCREASE DECREASE d. Determine the concentration (in ppm) of tartaric acid in the unknown solution.

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12. A standard solution with known concentrations of tartaric acid and fumaric acid was prepared and
measured using reversed phase liquid chromatography. The data for known concentrations is shown in
the table below:
Concentration
Peak Area
Sample
(ppm)
mobile phase
N/A
N/A
tartaric acid
455
369
fumaric acid
125
249
A second solution containing 5.00 mL of an unknown concentration of tartaric acid was combined with
10.0 ml of 81.0 ppm of fumaric acid and diluted with water to 100.00 ml in a 100.00 ml volumetric
flask. The chromatogram of this mixture produced peak areas of 244 and 125 for tartaric acid and
fumaric acid, respectively.
a. What type of cąlibration method is used in this analysis and how do you know?
b. In this analysis, the stationary phase is: Circle Oner:
POLAR
NO POLAR
c. By increasing the eluent strength of the mobile phase (making it more like the column polarity), how
would the retention time of the analyte change in the analysis? Circle Ones
INCREASE
DECREASE
d. Determine the concentration (in ppm) of tartaric acid in the unknown solution.
Transcribed Image Text:12. A standard solution with known concentrations of tartaric acid and fumaric acid was prepared and measured using reversed phase liquid chromatography. The data for known concentrations is shown in the table below: Concentration Peak Area Sample (ppm) mobile phase N/A N/A tartaric acid 455 369 fumaric acid 125 249 A second solution containing 5.00 mL of an unknown concentration of tartaric acid was combined with 10.0 ml of 81.0 ppm of fumaric acid and diluted with water to 100.00 ml in a 100.00 ml volumetric flask. The chromatogram of this mixture produced peak areas of 244 and 125 for tartaric acid and fumaric acid, respectively. a. What type of cąlibration method is used in this analysis and how do you know? b. In this analysis, the stationary phase is: Circle Oner: POLAR NO POLAR c. By increasing the eluent strength of the mobile phase (making it more like the column polarity), how would the retention time of the analyte change in the analysis? Circle Ones INCREASE DECREASE d. Determine the concentration (in ppm) of tartaric acid in the unknown solution.
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