12. A standard solution with known concentrations of tartaric acid and fumaric acid was prepared and measured using reversed phase liquld chromatography. The data for known concentrations is shown in the table below: Concentration (ppm) Peak Area Sample mobile phase N/A N/A tartaric acid 455 369 fumaric acid 125 249 A second solution containing 5.00 mL of an unknown concentration of tartaric acid was combined with 10.0 ml of 81.0 ppm of fumaric acid and diluted with water to 100.00 mL in a 100.00 ml volumetric flask. The chromatogram of this mixture produced peak areas of 244 and 125 for tartaric acid and fumaric acid, respectively. a. What type of calibration method is used in this analysis and how do you know? b. In this analysis, the stationary phase is: Circle Oner: POLAR NO POLAR C. By increasing the eluent strength of the mobile phase (making it more like the column polarity), how would the retention time of the analyte change in the analysis? Circle Ones INCREASE DECREASE d. Determine the concentration (in ppm) of tartaric acid in the unknown solution.

12. A standard solution with known concentrations of tartaric acid and fumaric acid was prepared and measured using reversed phase liquld chromatography. The data for known concentrations is shown in the table below: Concentration (ppm) Peak Area Sample mobile phase N/A N/A tartaric acid 455 369 fumaric acid 125 249 A second solution containing 5.00 mL of an unknown concentration of tartaric acid was combined with 10.0 ml of 81.0 ppm of fumaric acid and diluted with water to 100.00 mL in a 100.00 ml volumetric flask. The chromatogram of this mixture produced peak areas of 244 and 125 for tartaric acid and fumaric acid, respectively. a. What type of calibration method is used in this analysis and how do you know? b. In this analysis, the stationary phase is: Circle Oner: POLAR NO POLAR C. By increasing the eluent strength of the mobile phase (making it more like the column polarity), how would the retention time of the analyte change in the analysis? Circle Ones INCREASE DECREASE d. Determine the concentration (in ppm) of tartaric acid in the unknown solution.

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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