LO73 Determine which nucleotides are methylated based on a sequence that has been treated with sodium bisulfite. Which of the following is NOT true of bisulfite sequencing? All thymines revealed in DNA sequencing of bisulfite treated DNA were cytosines in the original, untreated DNA. ODNA is treated with sodium bisulfite, which chemically converts unmethylated cytosines to uracil. The sequence of genomic DNA treated with sodium bisulfite is compared to the sequence of untreated genomic DNA. Unmethylated cytosines are detected as thymine sequencing. Methylated cytosines are unaffected by sodium bisulfite. .

Human Anatomy & Physiology (11th Edition)
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Chapter1: The Human Body: An Orientation
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LO73 Determine which nucleotides are methylated based on a sequence that has been treated with sodium
bisulfite.
Which of the following is NOT true of bisulfite sequencing?
All thymines revealed in DNA sequencing of bisulfite treated DNA were cytosines in the original, untreated DNA.
ODNA is treated with sodium bisulfite, which chemically converts unmethylated cytosines to uracil.
The sequence of genomic DNA treated with sodium bisulfite is compared to the sequence of untreated genomic
DNA.
Unmethylated cytosines are detected as
duri sequencing.
Methylated cytosines are unaffected by sodium bisulfite.
→
Transcribed Image Text:LO73 Determine which nucleotides are methylated based on a sequence that has been treated with sodium bisulfite. Which of the following is NOT true of bisulfite sequencing? All thymines revealed in DNA sequencing of bisulfite treated DNA were cytosines in the original, untreated DNA. ODNA is treated with sodium bisulfite, which chemically converts unmethylated cytosines to uracil. The sequence of genomic DNA treated with sodium bisulfite is compared to the sequence of untreated genomic DNA. Unmethylated cytosines are detected as duri sequencing. Methylated cytosines are unaffected by sodium bisulfite. →
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