it into a glass tube filled with 40 ml of water (final volume). What is the amount of dye present in the You have a stock dye solution with a concentration of 0.25 mg/ml. You take 20 µl of this dye and place glass tube?
it into a glass tube filled with 40 ml of water (final volume). What is the amount of dye present in the You have a stock dye solution with a concentration of 0.25 mg/ml. You take 20 µl of this dye and place glass tube?
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
![**Question 8: Calculation of Dye in a Diluted Solution**
You have a stock dye solution with a concentration of 0.25 mg/ml. You take 20 µl of this dye and place it into a glass tube filled with 40 ml of water (final volume). What is the amount of dye present in the glass tube?
**Explanation:**
To find the amount of dye present in the tube, you first need to calculate the total amount of dye in the 20 µl taken from the stock solution.
1. **Determine the amount of dye in 20 µl:**
\[
\text{Amount of dye} = \text{concentration} \times \text{volume}
\]
Given:
- Concentration = 0.25 mg/ml
- Volume = 20 µl = 0.02 ml (since 1 ml = 1000 µl)
\[
\text{Amount of dye} = 0.25 \, \text{mg/ml} \times 0.02 \, \text{ml} = 0.005 \, \text{mg}
\]
2. **Understand dilution in the final volume:**
The total dye amount is 0.005 mg, which remains unchanged regardless of the addition of water. Therefore, the amount of dye present in the glass tube is 0.005 mg.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F18593a3a-b463-4aeb-a821-a472ffee6b36%2Fdb34fc2f-3233-47b0-aff4-3fe82a32f0a2%2Fip48ub6_processed.jpeg&w=3840&q=75)
Transcribed Image Text:**Question 8: Calculation of Dye in a Diluted Solution**
You have a stock dye solution with a concentration of 0.25 mg/ml. You take 20 µl of this dye and place it into a glass tube filled with 40 ml of water (final volume). What is the amount of dye present in the glass tube?
**Explanation:**
To find the amount of dye present in the tube, you first need to calculate the total amount of dye in the 20 µl taken from the stock solution.
1. **Determine the amount of dye in 20 µl:**
\[
\text{Amount of dye} = \text{concentration} \times \text{volume}
\]
Given:
- Concentration = 0.25 mg/ml
- Volume = 20 µl = 0.02 ml (since 1 ml = 1000 µl)
\[
\text{Amount of dye} = 0.25 \, \text{mg/ml} \times 0.02 \, \text{ml} = 0.005 \, \text{mg}
\]
2. **Understand dilution in the final volume:**
The total dye amount is 0.005 mg, which remains unchanged regardless of the addition of water. Therefore, the amount of dye present in the glass tube is 0.005 mg.
Expert Solution
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
This is a popular solution!
Trending now
This is a popular solution!
Step by step
Solved in 2 steps with 1 images
Recommended textbooks for you
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON