7. Short answer. Professor Forni has hired you as a research assistant and you need to make an X-gal staining solution for your sections containing 5mM potassium ferrocyanide, 5 mM potassium ferricyanide, 2 MM MgCls, 0.1% Tween and 0.1% BCIP. You need 50 ml. As you are getting ready you discover that your colleague has used most of the BCIP and you only have 150 ul of the BCIP left. Is it enough? Show your calculations. Short answer Profeccor Sai

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7. Short answer. Professor Forni has hired vou as a research assistant and you need to make an X-gal staining
solution for your sections containing 5mM potassium ferrocyanide, 5 mM potassium ferricyanide, 2 MM MgCl2,
0.1% Tween and 0.1% BCIP. You need 50 ml. As you are getting ready you discover that your colleague has
used most of the BCIP and you only have 150 ul of the BCIP left. Is it enough? Show your calculations.
8. Short answer. Professor Scimemi has accepted vou as a Master's student and you are involved in a project that
studies the cellular basis of neuropsychiatric diseases. For your electrophysiology recordings you have to make a
recording solution containing (in mM): 119 NaCI, 2.5 KCI, 2.5 CaCl2, 1 MgCl2, 26.2 NaHCO3, 1 NaH¿PO4, 22
glucose. You need 800 ml of it. How much NaCl and how much KCl do you need to weigh out? (MW NaCI is
58.44 g/mol; MW of KCI is 74.55 g/mol)
9. You are working as a research technician for Pfizer and in your lab, one solution of hydrochloric acid has a pH of
3.2, while another solution of sodium hydroxide has a pH of 9.7. Calculate the concentration of hydrogen ions in
each solution.
10. Choose the one answer that fits best. Water has a pH of 7. What does that mean?
a.
water has more H+ than OH-
water is acidic
the concentration of H+ in water is 10-7 M
the concentration of H+ in water is 7 nM
b.
с.
d.
e.
it is basic
33
Hasic Laboratory Skills Boot Camp I
Transcribed Image Text:7. Short answer. Professor Forni has hired vou as a research assistant and you need to make an X-gal staining solution for your sections containing 5mM potassium ferrocyanide, 5 mM potassium ferricyanide, 2 MM MgCl2, 0.1% Tween and 0.1% BCIP. You need 50 ml. As you are getting ready you discover that your colleague has used most of the BCIP and you only have 150 ul of the BCIP left. Is it enough? Show your calculations. 8. Short answer. Professor Scimemi has accepted vou as a Master's student and you are involved in a project that studies the cellular basis of neuropsychiatric diseases. For your electrophysiology recordings you have to make a recording solution containing (in mM): 119 NaCI, 2.5 KCI, 2.5 CaCl2, 1 MgCl2, 26.2 NaHCO3, 1 NaH¿PO4, 22 glucose. You need 800 ml of it. How much NaCl and how much KCl do you need to weigh out? (MW NaCI is 58.44 g/mol; MW of KCI is 74.55 g/mol) 9. You are working as a research technician for Pfizer and in your lab, one solution of hydrochloric acid has a pH of 3.2, while another solution of sodium hydroxide has a pH of 9.7. Calculate the concentration of hydrogen ions in each solution. 10. Choose the one answer that fits best. Water has a pH of 7. What does that mean? a. water has more H+ than OH- water is acidic the concentration of H+ in water is 10-7 M the concentration of H+ in water is 7 nM b. с. d. e. it is basic 33 Hasic Laboratory Skills Boot Camp I
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