Identify the open reading frame in the following DNA sequence, the protein that this gene encodes for, its function, and the source.GACTATATCCGGCGTATGAAGAAGGTTTCTACGCTTGACCTGTTGTTCGTTGCGATCATGGGTGTTTCGCCGGCCGCTTTTGCCGCCGACCTGATCGACGTGTCCAAACTCCCCAGCAAGGCTGCCCAGGGCGCGCCCGGCCCGGTCACCTTGCAAGCCGCGGTCGGCGCTGGCGGTGCCGACGAACTGAAAGCGATCCGCAGCACGACCCTGCCCAACGGCAAGCAGGTCACCCGCTACGAGCAATTCCACAACGGCGTACGGGTGGTCGGCGAAGCCATCACCGAAGTCAAGGGTCCCGGCAAGAGCGTGGCGGCGCAGCGCAGCGGCCATTTCGTCGCCAACATCGCTGCCGACCTGCCGGGCAGCACCACCGCGGCGGTATCCGCCGAGCAGGTGCTGGCCCAGGCCAAGAGCCTGAAGGCCCAGGGCCGCAAGACCGAGAATGACAAAGTGGAACTGGTGATCCGCCTGGGCGAGAACAACATCGCCCAACTGGTCTACAACGTCTCCTACCTGATTCCCGGCGAGGGACTGTCGCGGCCGCATTTCGTCATCGACGCCAAGACCGGCGAAGTGCTCGATCAGTGGGAAGGCCTGGCCCACGCCGAGGCGGGCGGCCCCGGCGGCAACCAGAAGATCGGCAAGTACACCTACGGTAGCGACTACGGTCCGCTGATCGTCAACGACCGCTGCGAGATGGACGACGGCAACGTCATCACCGTCGACATGAACAGCAGCACCGACGACAGCAAGACCACGCCGTTCCGCTTCGCCTGCCCGACCAACACCTACAAGCAGGTCAACGGCGCCTATTCGCCGCTGAACGACGCGCATTTCTTCGGCGGCGTGGTGTTCAAACTGTACCGGGACTGGTTCGGCACCAGCCCGCTGACCCACAAGCTGTACATGAAGGTGCACTACGGGCGCAGCGTGGAGAACGCCTACTGGGACGGCACGGCGATGCTCTTCGGCGACGGCGCCACCATGTTCTATCCGCTGGTGTCGCTGGACGTGGCGGCCCACGAGGTCAGCCACGGCTTCACCGAGCAGAACTCCGGGCTGATCTACCGCGGGCAATCAGGCGGAATGAACGAAGCGTTCTCCGACATGGCCGGCGAGGCTGCCGAGTTCTATATGCGCGGCAAGAACGACTTCCTGATCGGCTACGACATCAAGAAGGGCAGCGGTGCGCTGCGCTACATGGACCAGCCCAGCCGCGACGGGCGATCCATCGACAACGCGTCGCAGTACTACAACGGCATCGACGTGCACCACTCCAGCGGCGTGTACAACCGTGCGTTCTACCTGTTGGCCAATTCGCCGGGCTGGGATACCCGCAAGGCCTTCGAGGTGTTCGTCGACGCCAACCGCTACTACTGGACCGCCACCAGCAACTACAACAGCGGCGCCTGCGGGGTGATTCGCTCGGCGCAGAACCGCAACTACTCGGCGGCTGACGTCACCCGGGCGTTCAGCACCGTCGGCGTGACCTGCCCGAGCGCGTTGTAAThis question involves using a database. You will use a database for storage and mining of genome sequences. The procedure to identify the gene and the protein that it encodes is as follows:How to identify the start site for transcription? used EXPASY WEBsite translated this DNA sequence, as the IMAGE shown, there are genetic code is translated. i think I am doing wrong? 2. Click on the DNA sequence from the start site of transcription, select all of the sequence, and copy the sequenceShould I copy the letter which is highlitend RED, which is start site, and then past it to BLAST? ttacaacgcgctcgggcaggtcacgccgacggtgctgaacgcccgggtgacgtcagccgcL Q R A R AGH A D G AER PGD VS RcgagtagttgcggttctgcgccgagcgaatcaccccgcaggcgccgctgttgtagttgctRV VA V L R R A N H PA GA A V V V AggtggcggtccagtagtagcggttggcgtcgacgaacacctcgaaggccttgcgggtatcGG G P V VA V G V DEH LEG LAGIccagcccggcgaattggccaacaggtagaacgcacggttgtacacgccgctggagtggtgPARR I G Q Q VERT V V HA A G V VcacgtcgatgccgttgtagtactgcgacgcgttgtcgatggatcgcccgtcgcggctgggHV DA V V V L R R V VDG SPVA A GctggtccatgtagcgcagcgcaccgctgcccttcttgatgtcgtagccgatcaggaagtcLV HVA QRTA A LLD V VAD QE VgttcttgccgcgcatatagaactcggcagcctcgccggccatgtcggagaacgcttcgttVLA A HI E L GS LA GHV GERF VcattccgcctgattgcccgcggtagatcagcccggagttctgctcggtgaagccgtggctHSA - L P A V D Q P G V L L G E A VAgacctcgtgggccgccacgtccagcgacaccagcggatagaacatggtggcgccgtcgccDL VG RH V Q R H Q R I E H G G A VAgaagagcatcgccgtgccgtcccagtaggcgttctccacgctgcgcccgtagtgcaccttEEHR RAV P V G V LHA A P V V HLcatgtacagcttgtgggtcagcgggctggtgccgaaccagtcccggtacagtttgaacacHV Q L V G Q R A GAEP V P V Q FEHcacgccgccgaagaaatgcgcgtcgttcagcggcgaataggcgccgttgacctgcttgtaHA A E E MR V V Q R R I G A V D L L VggtgttggtcgggcaggcgaagcggaacggcgtggtcttgctgtcgtcggtgctgctgttGV GRAGE AERR GLA V VGA A VcatgtcgacggtgatgacgttgccgtcgtccatctcgcagcggtcgttgacgatcagcggHV D G D D VA V V HLA A V V D D QRaccgtagtcgctaccgtaggtgtacttgccgatcttctggttgccgccggggccgcccgcTV VAT V G VLA D L L VA A G A ARctcggcgtgggccaggccttcccactgatcgagcacttcgccggtcttggcgtcgatgacLGV G Q AFP LI E H F A G L G V D Dgaaatgcggccgcgacagtccctcgccgggaatcaggtaggagacgttgtagaccagttg 5https://web.expasy.org/translate/Translate is a tool which allows the translation of a nucleotide (DNA/RNA) sequence to a protein sequence.DNA or RNA sequencegactatatccggcgtatgaagaaggtttctacgcttgacctgttgttcgttgcgatcatgggtgtttcgccggccgcttttgccgccgacctgatcgacgtgtccaaactccccagcaaggctgcccagggcgcgcccggcccggtcaccttgcaagccgcggtcggcgctggcggtgccgacgaactgaaagcgatccgcagcacgaccctgcccaacggcaagcaggtcacccgctacgagcaattccacaacggcgtacgggtggtcggcgaagccatcaccgaagtcaagggtcccggcaagagcgtggcggcgcagcgcagcggccatttcgtcgccaacatcgctgccgacctgccgggcagcaccaccgcggcggtatccgccgagcaggtgctggcccGenetic codes - See NCBI's genetic codesStandardresetResults of translationTranslate toolTRANSLATE!.3'5' Frame 1Output formatO Verbose: Met, Stop, spaces between residuesO Compact: M, -, no spacesO Includes nucleotide sequenceIncludes nucleotide sequence, no spacesOpen reading frames are highlighted in red• Select your initiator on one of the following frames to retrieve youramino acid sequenceDNA strandsO forward ✔reverseDownload all the translated framesttacaacgcgctcgggcaggtcacgccgacggtgctgaacgcccgggtgacgtcagccgcLQRA RAG HAD GAER PGD VS RcgagtagttgcggttctgcgccgagcgaatcaccccgcaggcgccgctgttgtagttgctR V 17 A V T R R A N H PAG A ADV V A

The genome or genomic data of an organism refers to the complete set of its hereditary material…

Answered: How to identify the start site for…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Question

Identify the open reading frame in the following DNA sequence, the protein that this gene encodes for, its function, and the source.

GACTATATCCGGCGTATGAAGAAGGTTTCTACGCTTGACCTGTTGTTCGTTGCGATCATGGGTGTTTCGC
CGGCCGCTTTTGCCGCCGACCTGATCGACGTGTCCAAACTCCCCAGCAAGGCTGCCCAGGGCGCGCCCGG
CCCGGTCACCTTGCAAGCCGCGGTCGGCGCTGGCGGTGCCGACGAACTGAAAGCGATCCGCAGCACGACC
CTGCCCAACGGCAAGCAGGTCACCCGCTACGAGCAATTCCACAACGGCGTACGGGTGGTCGGCGAAGCCA
TCACCGAAGTCAAGGGTCCCGGCAAGAGCGTGGCGGCGCAGCGCAGCGGCCATTTCGTCGCCAACATCGC
TGCCGACCTGCCGGGCAGCACCACCGCGGCGGTATCCGCCGAGCAGGTGCTGGCCCAGGCCAAGAGCCTG
AAGGCCCAGGGCCGCAAGACCGAGAATGACAAAGTGGAACTGGTGATCCGCCTGGGCGAGAACAACATCG
CCCAACTGGTCTACAACGTCTCCTACCTGATTCCCGGCGAGGGACTGTCGCGGCCGCATTTCGTCATCGA
CGCCAAGACCGGCGAAGTGCTCGATCAGTGGGAAGGCCTGGCCCACGCCGAGGCGGGCGGCCCCGGCGGC
AACCAGAAGATCGGCAAGTACACCTACGGTAGCGACTACGGTCCGCTGATCGTCAACGACCGCTGCGAGA
TGGACGACGGCAACGTCATCACCGTCGACATGAACAGCAGCACCGACGACAGCAAGACCACGCCGTTCCG
CTTCGCCTGCCCGACCAACACCTACAAGCAGGTCAACGGCGCCTATTCGCCGCTGAACGACGCGCATTTC
TTCGGCGGCGTGGTGTTCAAACTGTACCGGGACTGGTTCGGCACCAGCCCGCTGACCCACAAGCTGTACA
TGAAGGTGCACTACGGGCGCAGCGTGGAGAACGCCTACTGGGACGGCACGGCGATGCTCTTCGGCGACGG
CGCCACCATGTTCTATCCGCTGGTGTCGCTGGACGTGGCGGCCCACGAGGTCAGCCACGGCTTCACCGAG
CAGAACTCCGGGCTGATCTACCGCGGGCAATCAGGCGGAATGAACGAAGCGTTCTCCGACATGGCCGGCG
AGGCTGCCGAGTTCTATATGCGCGGCAAGAACGACTTCCTGATCGGCTACGACATCAAGAAGGGCAGCGG
TGCGCTGCGCTACATGGACCAGCCCAGCCGCGACGGGCGATCCATCGACAACGCGTCGCAGTACTACAAC
GGCATCGACGTGCACCACTCCAGCGGCGTGTACAACCGTGCGTTCTACCTGTTGGCCAATTCGCCGGGCT
GGGATACCCGCAAGGCCTTCGAGGTGTTCGTCGACGCCAACCGCTACTACTGGACCGCCACCAGCAACTA
CAACAGCGGCGCCTGCGGGGTGATTCGCTCGGCGCAGAACCGCAACTACTCGGCGGCTGACGTCACCCGG
GCGTTCAGCACCGTCGGCGTGACCTGCCCGAGCGCGTTGTAA

This question involves using a database. You will use a database for storage and mining of genome sequences. The procedure to identify the gene and the protein that it encodes is as follows:

How to identify the start site for transcription?

used EXPASY WEBsite translated this DNA sequence, as the IMAGE shown, there are genetic code is translated. i think I am doing wrong?

2. Click on the DNA sequence from the start site of transcription, select all of the sequence, and copy the sequence

Should I copy the letter which is highlitend RED, which is start site, and then past it to BLAST?

ttacaacgcgctcgggcaggtcacgccgacggtgctgaacgcccgggtgacgtcagccgc
L Q R A R AGH A D G AER PGD VS R
cgagtagttgcggttctgcgccgagcgaatcaccccgcaggcgccgctgttgtagttgct
RV VA V L R R A N H PA GA A V V V A
ggtggcggtccagtagtagcggttggcgtcgacgaacacctcgaaggccttgcgggtatc
GG G P V VA V G V DEH LEG LAGI
ccagcccggcgaattggccaacaggtagaacgcacggttgtacacgccgctggagtggtg
PARR I G Q Q VERT V V HA A G V V
cacgtcgatgccgttgtagtactgcgacgcgttgtcgatggatcgcccgtcgcggctggg
HV DA V V V L R R V VDG SPVA A G
ctggtccatgtagcgcagcgcaccgctgcccttcttgatgtcgtagccgatcaggaagtc
LV HVA QRTA A LLD V VAD QE V
gttcttgccgcgcatatagaactcggcagcctcgccggccatgtcggagaacgcttcgtt
VLA A HI E L GS LA GHV GERF V
cattccgcctgattgcccgcggtagatcagcccggagttctgctcggtgaagccgtggct
HSA - L P A V D Q P G V L L G E A VA
gacctcgtgggccgccacgtccagcgacaccagcggatagaacatggtggcgccgtcgcc
DL VG RH V Q R H Q R I E H G G A VA
gaagagcatcgccgtgccgtcccagtaggcgttctccacgctgcgcccgtagtgcacctt
EEHR RAV P V G V LHA A P V V HL
catgtacagcttgtgggtcagcgggctggtgccgaaccagtcccggtacagtttgaacac
HV Q L V G Q R A GAEP V P V Q FEH
cacgccgccgaagaaatgcgcgtcgttcagcggcgaataggcgccgttgacctgcttgta
HA A E E MR V V Q R R I G A V D L L V
ggtgttggtcgggcaggcgaagcggaacggcgtggtcttgctgtcgtcggtgctgctgtt
GV GRAGE AERR GLA V VGA A V
catgtcgacggtgatgacgttgccgtcgtccatctcgcagcggtcgttgacgatcagcgg
HV D G D D VA V V HLA A V V D D QR
accgtagtcgctaccgtaggtgtacttgccgatcttctggttgccgccggggccgcccgc
TV VAT V G VLA D L L VA A G A AR
ctcggcgtgggccaggccttcccactgatcgagcacttcgccggtcttggcgtcgatgac
LGV G Q AFP LI E H F A G L G V D D
gaaatgcggccgcgacagtccctcgccgggaatcaggtaggagacgttgtagaccagttg

5
https://web.expasy.org/translate/
Translate is a tool which allows the translation of a nucleotide (DNA/RNA) sequence to a protein sequence.
DNA or RNA sequence
gactatatccggcgtatgaagaaggtttctacgcttgacctgttgttcg
ttgcgatcatgggtgtttcgccggccgcttttgccgccgacctgatcg
acgtgtccaaactccccagcaaggctgcccagggcgcgcccggc
ccggtcaccttgcaagccgcggtcggcgctggcggtgccgacgaa
ctgaaagcgatccgcagcacgaccctgcccaacggcaagcaggt
cacccgctacgagcaattccacaacggcgtacgggtggtcggcg
aagccatcaccgaagtcaagggtcccggcaagagcgtggcggc
gcagcgcagcggccatttcgtcgccaacatcgctgccgacctgcc
gggcagcaccaccgcggcggtatccgccgagcaggtgctggccc
Genetic codes - See NCBI's genetic codes
Standard
reset
Results of translation
Translate tool
TRANSLATE!
.
3'5' Frame 1
Output format
O Verbose: Met, Stop, spaces between residues
O Compact: M, -, no spaces
O Includes nucleotide sequence
Includes nucleotide sequence, no spaces
Open reading frames are highlighted in red
• Select your initiator on one of the following frames to retrieve your
amino acid sequence
DNA strands
O forward ✔reverse
Download all the translated frames
ttacaacgcgctcgggcaggtcacgccgacggtgctgaacgcccgggtgacgtcagccgc
LQRA RAG HAD GAER PGD VS R
cgagtagttgcggttctgcgccgagcgaatcaccccgcaggcgccgctgttgtagttgct
R V 17 A V T R R A N H PAG A A
D
V V A

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Follow-up Question

I am more confused. how about we start from begining, you post answers on here, and then we go from there?

1. Identify the open reading frame in the following DNA sequence, the protein that this gene encodes for, its function, and the source.

2. "Look carefully at the DNA sequence and identify the start site for transcription"

Click on the DNA sequence from the start site of transcription, select all of the sequence, and copy the sequence.
Go to the National Center for Biotechnology Information (NCBI) website http://www.ncbi.nlm.nih.gov/. Click on BLAST on the right-hand side under “Popular Resources.” BLAST is a program that will allow you to find the protein sequence for the DNA sequence (gene) you submit. Next click on blastx (translated nucleotide protein).
Paste the DNA sequence into the box under “Entry Query Sequence.” Scroll down and click BLAST. The search may take a few seconds; the page will keep updating until the search is completed. You do not need to enter any parameters in the boxes before you click BLAST.
When the search is complete, you will have a figure showing the most homologous results or “sequences producing significant alignments”; and, after that, a list of what these proteins are. Your protein will be the first one on the list. You can click on the accession number or sequence identifier information to bring up more information. You should be able to find the name, function, size (number of amino acids), and source (name of the organism) for the protein. Your answer should include the:amino acid sequence of the protein
size of the protein: NCBI reference sequence WP_0031138351 CONSISTE 498 amino acids long
identity of the protein: multispecies, M4 family elastase LasB ( Pseudomonas)
function of the protein (you may need to do additional online research to find this)
bacteria Pseudomonas aeruginosa. Its main function is to break down elastin which is a component of the connect tissue in human. This enzyme helps the bacteria to invade tissues and cause infections by degrading elastin in the host tissue. LasB has also been shown to cleave a variety of other host proteins including immunoglobulins, complement components, and coagulation factors, which further contributes to the pathogenesis of Pseudomonas infections. In addition to its virulence factors, LasB also has industrial and biotechnological applications due to its proteolytic activity.(PubMed)

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Follow-up Question

Identify the open reading frame in the following DNA sequence

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Follow-up Question

Can you show me the step how you get the amino acid sequence?

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Follow-up Question

what is the amino acid squence of the protein?

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