How will you prepare 750 ml of the digestion buffer. Show all steps and calculations and remember to explain how exactly you will make it up. You areSupplied with the following:NaCI (Mr= 58.443 9/mol)250 mM Tri-Cl, pH 8 solution (1 litre)EDTA, Sodium salt CMr= 380 2qlmo/)29.13% Sodium dodecy 1 sulfate solutionsolution Ci50Proteinase Kdi ssolvedmgin ImlYou need adigestion buffer Consisting of the followingstron23.5mM.Nacy750 uM Tris-Ccl.pH 838 mMEDTA, pHl 812% sodium1 75 mg/ml proteinasé k.dodecly Sulfate1.75

Digestion buffers are used to digest or break open the cells. It is composed of buffering salt…

Answered: How will you prepare 750 ml of the…

How will you prepare 750 ml of the digestion buffer. Show all steps and calculations and remember to explain how exactly you will make it up.

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question

How will you prepare 750 ml of the digestion buffer. Show all steps and calculations and remember to explain how exactly you will make it up.

You are
Supplied with the following:
NaCI (Mr= 58.443 9/mol)
250 mM Tri-Cl, pH 8 solution (1 litre)
EDTA, Sodium salt CMr= 380 2qlmo/)
29.
13% Sodium dodecy 1 sulfate solution
solution Ci50
Proteinase K
di ssolved
mg
in Iml
You need a
digestion buffer Consisting of the following
stron
23.5mM.Nacy
750 uM Tris-Ccl.pH 8
38 mM
EDTA, pHl 8
12% sodium
1 75 mg/ml proteinasé k.
dodecly Sulfate
1.75

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