Good Day I have this practise question but i have not idea how to solve it. The quesion is as follows: How will you prepare 500ml of digestion buffer? And they give me this information: You are supplied with the following: NaCl (Mr = 58.443 g/mol) 2.5 M Tris-Cl, pH 8 oplossing / solution (1 Litre) EDTA, sodium salt (Mr = 380.2 g/mol) 10 % Sodium dodecyl sulfate solution Proteinase K solution (50 mg dissolved in 1 ml ddH2O) You need a digestion buffer consisting of the following: 15 mM NaCl 75 mM Tris-Cl, pH 8 16 mM EDTA, pH 8 0.8 % Sodium dodecyl sulfate 0.75 mg/ml proteinase K
Nucleotides
It is an organic molecule made up of three basic components- a nitrogenous base, phosphate,and pentose sugar. The nucleotides are important for metabolic reactions andthe formation of DNA (deoxyribonucleic acid) and RNA (ribonucleic acid).
Nucleic Acids
Nucleic acids are essential biomolecules present in prokaryotic and eukaryotic cells and viruses. They carry the genetic information for the synthesis of proteins and cellular replication. The nucleic acids are of two types: deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). The structure of all proteins and ultimately every biomolecule and cellular component is a product of information encoded in the sequence of nucleic acids. Parts of a DNA molecule containing the information needed to synthesize a protein or an RNA are genes. Nucleic acids can store and transmit genetic information from one generation to the next, fundamental to any life form.
Good Day
I have this practise question but i have not idea how to solve it. The quesion is as follows:
How will you prepare 500ml of digestion buffer?
And they give me this information:
You are supplied with the following:
NaCl (Mr = 58.443 g/mol)
2.5 M Tris-Cl, pH 8 oplossing / solution (1 Litre)
EDTA, sodium salt (Mr = 380.2 g/mol)
10 % Sodium dodecyl sulfate solution
Proteinase K solution (50 mg dissolved in 1 ml ddH2O)
You need a digestion buffer consisting of the following:
15 mM NaCl
75 mM Tris-Cl, pH 8
16 mM EDTA, pH 8
0.8 % Sodium dodecyl sulfate
0.75 mg/ml proteinase K
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