Lambda Digest Volume to add Tube 2 (EcoRI, HindIII) Tube 1 Mass or Conc. Tube 3 Tub mponent Concentration (control) required (EcoRI) (Hine 0.5 μgμL 1 ug versal 10X 1X er

Curren'S Math For Meds: Dosages & Sol
11th Edition
ISBN:9781305143531
Author:CURREN
Publisher:CURREN
Chapter5: Unit, Percentage, Milliequivalent, Ratio, And Household Measures
Section: Chapter Questions
Problem 1.2P
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Predict the result

Lambda Digest
Volume to add
Tube 1
Tube 2
Mass or Conc.
Tube 3
Tube 4
(ECORI,
HindIII)
Component
Concentration
(control)
required
(EcoRI)
(HindIII)
DNA
0.5 ug/uL
1 µg
Universal
buffer
10X
Water
EcoRI
0.5 units/uL
1 unit
none
Hind III
1 unit
none
0.5 units/uL
ТОTAL
20 µL
20 µL
20 µL
20 µL
PGLO digest
Volume to add
Tube 1
Tube 2
Mass or Conc.
Tube 3
Tube 4
(negative (EcoRI,
control)
НindШ)
Component
Concentration
required
(EcoRI)
(HindШ)
DNA
unknown
1 ug
Universal
1X
buffer
10X
Water
1 unit
none
0.5 units/uL
Hind III
1 unit
none
0.5 units/uL
ТОTAL
20 µL
20 µL
20 µL
20 µL
Transcribed Image Text:Lambda Digest Volume to add Tube 1 Tube 2 Mass or Conc. Tube 3 Tube 4 (ECORI, HindIII) Component Concentration (control) required (EcoRI) (HindIII) DNA 0.5 ug/uL 1 µg Universal buffer 10X Water EcoRI 0.5 units/uL 1 unit none Hind III 1 unit none 0.5 units/uL ТОTAL 20 µL 20 µL 20 µL 20 µL PGLO digest Volume to add Tube 1 Tube 2 Mass or Conc. Tube 3 Tube 4 (negative (EcoRI, control) НindШ) Component Concentration required (EcoRI) (HindШ) DNA unknown 1 ug Universal 1X buffer 10X Water 1 unit none 0.5 units/uL Hind III 1 unit none 0.5 units/uL ТОTAL 20 µL 20 µL 20 µL 20 µL
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