PGLO digest Volume to add Tube 1 Tube 2 Mass or Conc. Tube 3 Tube 4 (negative control) Сomponent Concentration required (EcoRI, HindlII) (EcoRI) (HindlII) DNA unknown I 18 Universal buffer 10X IX Water EcoRI 0.5 units/ul. I unit none Hind III I unit none 05 units/ul 20 ul. ТОTAL 20 ul. 20 ul. 20 ul.

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
icon
Related questions
Question

Predict the result by fill up the number

PGLO digest
Volume to add
Tube 1
Tube 2
Mass or Conc.
Tube 3
Tube 4
(negative
control)
Component
Сoвсentration
(EcoRI,
required
(EcoRI)
(HindllI)
Hindlll)
DNA
unknown
I 18
Universal
IX
buffer
10X
Water
EcoRI
0.5 units/ul
I unit
none
Hind III
I unit
none
05 units/ul
ТОTAL
20 μL
20 ul.
20 ul.
20 ul.
Transcribed Image Text:PGLO digest Volume to add Tube 1 Tube 2 Mass or Conc. Tube 3 Tube 4 (negative control) Component Сoвсentration (EcoRI, required (EcoRI) (HindllI) Hindlll) DNA unknown I 18 Universal IX buffer 10X Water EcoRI 0.5 units/ul I unit none Hind III I unit none 05 units/ul ТОTAL 20 μL 20 ul. 20 ul. 20 ul.
Expert Solution
steps

Step by step

Solved in 2 steps

Blurred answer
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education