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4. Suppose you are studying gene X in yeast using four different strains. Strain 1 is haploid and has the wild type allele of gene X. Strain 2 is haploid and has a missense mutation halfway through the coding sequence of gene Х. Strain 3 is haploid and has a nonsense mutation halfway through the coding sequence of , Х. gene Strain 4 is diploid and is heterozygous for the nonsense mutation in gene X (i.e., it carries the wild type allele of gene X on one chromosome and the nonsense allele of gene X on the other homologue). You have a DNA probe that will hybridize with gene X and its mRNA transcript on southern and northern blots, respectively. You also have an antibody that will bind to the protein encoded by gene X on western blots. You extract DNA, RNA, and protein from the four strains described above and do southern, northern, and western blot analyses to compare gene X, its mRNA transcript, and its protein in these strains. Please draw the bands you expect to see. The positions of the wild type bands are indicated. 3 points: +1 point for each completely correct blot. 1 2 3 4 1 2 3 4 1 3 4 Southern Blot Northern Blot Western Blot

4. Suppose you are studying gene X in yeast using four different strains. Strain 1 is haploid and has the wild type allele of gene X. Strain 2 is haploid and has a missense mutation halfway through the coding sequence of gene Х. Strain 3 is haploid and has a nonsense mutation halfway through the coding sequence of , Х. gene Strain 4 is diploid and is heterozygous for the nonsense mutation in gene X (i.e., it carries the wild type allele of gene X on one chromosome and the nonsense allele of gene X on the other homologue). You have a DNA probe that will hybridize with gene X and its mRNA transcript on southern and northern blots, respectively. You also have an antibody that will bind to the protein encoded by gene X on western blots. You extract DNA, RNA, and protein from the four strains described above and do southern, northern, and western blot analyses to compare gene X, its mRNA transcript, and its protein in these strains. Please draw the bands you expect to see. The positions of the wild type bands are indicated. 3 points: +1 point for each completely correct blot. 1 2 3 4 1 2 3 4 1 3 4 Southern Blot Northern Blot Western Blot

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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4. Suppose you are studying gene X in yeast using four different strains. Strain 1 is haploid and has the wild type allele of gene X. Strain 2 is haploid and has a missense mutation halfway through the coding sequence of gene Х. Strain 3 is haploid and has a nonsense mutation halfway through the coding sequence of gene Х. Strain 4 is diploid and is heterozygous for the nonsense mutation in gene X (i.e., it carries the wild type allele of gene X on one chromosome and the nonsense allele of gene X on the other homologue). You have a DNA probe that will hybridize with gene X and its mRNA transcript on southern and northern blots, respectively. You also have an antibody that will bind to the protein encoded by gene X on western blots. You extract DNA, RNA, and protein from the four strains described above and do southern, northern, and western blot analyses to compare gene X, its mRNA transcript, and its protein in these strains. Please draw the bands you expect to see. The positions of the wild type bands are indicated. 3 points: +1 point for each completely correct blot. 1 3 4 1 2 3 4 1 2 3 4 ern Blot Northern Blot Western Blot | |
Transcribed Image Text:4. Suppose you are studying gene X in yeast using four different strains. Strain 1 is haploid and has the wild type allele of gene X. Strain 2 is haploid and has a missense mutation halfway through the coding sequence of gene Х. Strain 3 is haploid and has a nonsense mutation halfway through the coding sequence of gene Х. Strain 4 is diploid and is heterozygous for the nonsense mutation in gene X (i.e., it carries the wild type allele of gene X on one chromosome and the nonsense allele of gene X on the other homologue). You have a DNA probe that will hybridize with gene X and its mRNA transcript on southern and northern blots, respectively. You also have an antibody that will bind to the protein encoded by gene X on western blots. You extract DNA, RNA, and protein from the four strains described above and do southern, northern, and western blot analyses to compare gene X, its mRNA transcript, and its protein in these strains. Please draw the bands you expect to see. The positions of the wild type bands are indicated. 3 points: +1 point for each completely correct blot. 1 3 4 1 2 3 4 1 2 3 4 ern Blot Northern Blot Western Blot | |
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