how to design an experiment to test weather the mutation is on enhancer, promoter, or protein-coding region?
Q: What is linker scanning mutagenesis?
A: Answer: Introduction: Old-style approaches, containing linker-scanning mutagenesis includes…
Q: Describe the difference between a transcriptional fusion and a translational reporter gene fusion.…
A: Transcription fusion means cloning the promoter of interest, upstream of any transcription unit,…
Q: How do you clone a 4 kb promoter from a genomic cell preparation?
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Q: What color is the alpha gene spot on the microarray? Answer: Yellow please explain why is the…
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Q: What is a simple way of making a knockout mouse in terms of DNA recombinant technology?
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Q: You have discovered a new bHLH gene and would like to use ChIP-Seq to learn more about it's role in…
A: A basic Helix-Loop-Helix Domain (bHLH) is a very common domain found in proteins that act as…
Q: During experimental RNAi, how does the researcher affect expression of a target gene? Group of…
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Q: A series of exonuclease deletions were used to study the promoter of the rice hemA gene, giving the…
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Q: here is a hypothetical gene related to the nervous system of Drosophila. Describe all the methods,…
A: In situ hybridization (ISH) is a type of hybridization that uses a labeled complementary DNA, RNA or…
Q: When Laybourne and Kadonaga studied the effects of histone proteins on eukaryotic transcription…
A: a) To clarify the mechanism of transcriptional repression by the nucleosomal cores they considered…
Q: You are interested in finding out in which organ GTF2H5 is highly expressed experimentally. Describe…
A: Recombinant DNA also written as rDNA is formed by joining or combining the DNA from different…
Q: Enhancers are sequences that affect the initiation of the transcription of genes that are hundreds…
A: apart from the regular looping mechanism, enhancers are also capable of 3 more mechanisms that can…
Q: Which of the following methods is utilized by eukaryotes to control their gene expression that is…
A: Gene regulation is the process of controlled expressions of specific gene from the entire genome of…
Q: how to design an experiment to test whether the mutation is on enhancer region or not ?
A: The regulatory DNA sequences that increase the transcription of a target gene are known as…
Q: Scientists carried out a microarray analysis to compare the gene expression of normal pancreatic…
A: Scientists can use microarray analysis to compare the gene expression of normal cells to that of…
Q: What are the functions of transcriptional activator proteins and repressor proteins? Explain how…
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Q: What is the difference between a promoter proximal element and a distal enhancer? What are the…
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Q: Before inserting the blunt-ended PCR amplicon to a dephosphorylated vector, the amplicon is treated…
A: PCR or polymerase chain reaction is a technique used to amplify a given set of DNA fragment using…
Q: You would like to study the effect of GeneX in the developing mouse liver. However, your supervisor…
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Q: What is the function of RAG1? What will happen if RAG1's function is lost due to mutations?
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Q: In the module, you have learned about P-element mediated transgenesis in Drosophila and the concept…
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Q: Microarrays can be used to determine relative levels of gene expression. In one type of microarray,…
A: DNA microarray is useful in detecting specific genes of concern in the pool of mRNAs extracted from…
Q: What are site-recombinases? Describe in detail how cre- recombinase can be used to decipher the…
A: Genetic recombination is the transfer of genetic material between organisms, resulting in offspring…
Q: In the replica plate experiment, it shows that mutations are random. However, certain environmental…
A: Introduction :- Mutations are changes that occur in the DNA sequence of an organism. They can happen…
how to design an experiment to test weather the mutation is on enhancer, promoter, or protein-coding region?
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- There is a hypothetical gene related to the nervous system of Drosophila. Describe all the methods, steps, and key substances you need to obtain to use the following techniques in experimental design to study the gene: - In situ hybridization (to find the mRNA) - Immunohistochemistry (to find the protein) - CRISPR-Cas9 (for loss of function) - Expression vector (for gain of function)You have discovered a new bHLH gene and would like to use ChIP-Seq to learn more about it's role in gene regulation. Describe the experiment you would perform, include your expected results.Consider the microarray in Figure 20.12. If a sample from normal tissue is labeled with a green fluorescent dye and a sample from cancerous tissue is labeled red, what color spots would represent genes you would be interested in if you were studying cancer? Explain.
- In the module, you have learned about P-element mediated transgenesis in Drosophila and the concept of using transgenes to rescue mutant phenotypes. In the figure below, you will see a wild type fly with its natural eye colour and three mutants with their eye colours changed to vermillion, white and rosy, respectively. A schematic of P-element mediated transgenesis (as shown in the lectures) is also included in the figure. Please inspect the schematic carefully and choose which of the following statements is true: I. Injection of the white experimental transgene into the vermillion mutant embryo will not change the vermillion mutant phenotype II. Injection of the white experimental transgene in the rosy mutant embryo will change rosy eye colour to red (wild type) III. Injection of the white experimental transgene in the white mutant embryo will not change the white mutant phenotype IV. Injection of the white experimental transgene in the rosy mutant…How can DNA microarrays be used to determine which genes of a bacterial pathogen are expressed both inside and outside of a host cell?What are site-recombinases? Describe in detail how cre- recombinase can be used to decipher the roles of specific genes and proteins in complex multicellular organisms? Explain how the cre-lox system can be used to examine the role of a particular gene in a specific type of tissue?
- Which of the following methods is utilized by eukaryotes to control their gene expression that is different from the type of control found in bacteria? a) control of chromatic remodeling. b) control of RNA splicing. c) transcriptional control. d) control of both RNA splicing and chromatin remodeling.A series of exonuclease deletions were used to study the promoter of the rice hemA gene, giving the results shown below (the intact promoter is on top). Based on these results, what conclusions could you draw from each of the deletion construct, and what do you know about the nature of the promoter? Relative activity -500 Reporter gene 100% Reporter gene 180% -350 -250- Reporter gene 180% 75% -150 Reporter gene 45% Reporter gene -100 0% - 8 Reporter geneScientists carried out a microarray analysis to compare the gene expression of normal pancreatic cells to that of cancer cells from a person with pancreatic cancer. The scientists labeled the cDNA from the normal pancreatic cells with green fluorescent nucleotides. They labeled the cDNA from the cancer cells with red fluorescent nucleotides. The two cDNAs were mixed and allowed to hybridize to a microarray. Less p53 activity is found in cancer pancreatic cells than normal cells. What color would the spot for the p53 gene be on the microarray? Red Green Yellow Black
- Scientists carried out a microarray analysis to compare the gene expression of normal liver cells to that of cancer cells from a person with liver cancer. The scientists labeled the cDNA from the normal pancreatic cells with green fluorescent nucleotides. They labeled the cDNA from the cancer cells with red fluorescent nucleotides. The two cDNAs were mixed and allowed to hybridize to a microarray. Normal liver cells =Green Cancer cells =Red The DNA in that spot is the same in cancer cells and normal cells =Yellow Question: Gene alpha is turned on in all liver cells. In the cancer sample there is a nonsense mutation in this gene such that the protein terminates translation early but transcription is unaffected. What color is the alpha gene spot on the microarray? Answer: Yellow please explain why is the answer yellow?You would like to study the effect of GeneX in the developing mouse liver. However, your supervisor says that GeneX knockouts (loss of function mutants) are embryonic lethal. What technique could you use to knockout GeneX only in the liver without causing embryonic lethality. Describe how you would do this experimentWhen Laybourne and Kadonaga studied the effects of histone proteins on eukaryotic transcription using an in vitro transcription assay explain why: a) they used two different DNA templates that contained different promoter structures. b) when they included both activator protein and histones, they always added the histone proteins before adding the activator to the transcription assay mixture. (Ctri) -
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