Gukde RNA Gulde RNA O The CRISPR-Case system is made up of gulde RNA molecitos and Case proteins. Case - PERV DNA The pol gene within a double-strandod PERV DNA Sequence is targotod within the pig gonome. The guide RNAS bring Casa to thoir targot DNA Sequences via complementary basé-pairing. pol Case cuts through both strands of the target DNA in the pig chromosome, and the CRISPR Case complox is releasod. O During nomal repair processes, the DNA soquonces betwoon the cut sites are removed and the romaining DNA is relinked, "knocking out" most of the pol gene. What remains of pol is not functional.

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
icon
Related questions
Question

Q1: What common mechanism is employed by the guide RNA to find its target DNA sequence? Q2: How many strands of DNA must Cas9 cut to be effective? Q3: Does Cas9 also cause the deletion of DNA from the genome?

Gukde RNA
Gulde RNA
O The CRISPR-Case
system is made up of
gulde RNA molecitos
and Case proteins.
Case
- PERV DNA
The pol gene within a
double-strandod
PERV DNA Sequence
is targotod within the
pig gonome. The
guide RNAS bring
Casa to thoir targot
DNA Sequences via
complementary
basé-pairing.
pol
Case cuts through
both strands of the
target DNA in the pig
chromosome, and
the CRISPR Case
complox is releasod.
O During nomal repair
processes, the DNA
soquonces betwoon
the cut sites are
removed and the
romaining DNA is
relinked, "knocking
out" most of the pol
gene. What remains
of pol is not
functional.
Transcribed Image Text:Gukde RNA Gulde RNA O The CRISPR-Case system is made up of gulde RNA molecitos and Case proteins. Case - PERV DNA The pol gene within a double-strandod PERV DNA Sequence is targotod within the pig gonome. The guide RNAS bring Casa to thoir targot DNA Sequences via complementary basé-pairing. pol Case cuts through both strands of the target DNA in the pig chromosome, and the CRISPR Case complox is releasod. O During nomal repair processes, the DNA soquonces betwoon the cut sites are removed and the romaining DNA is relinked, "knocking out" most of the pol gene. What remains of pol is not functional.
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 2 steps

Blurred answer
Knowledge Booster
Genomics
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biochemistry and related others by exploring similar questions and additional content below.
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON