Briefly describe three stages of transcription.  How is transcription initiated? How is transcription terminated?

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Briefly describe three stages of transcription.  How is transcription initiated? How is transcription terminated?

 

Stop site
on template
strand
RNA polymerase
Start site
on template
strand
INITIATION
1 Polymerase binds to
promoter sequence
in duplex DNA.
"Closed complex"
3'
Promoter
2 Polymerase melts
duplex DNA near
transcription start site,
forming a transcription
bubble.
5-
3'
Transcription
bubble
"Open complex"
Initial rNTPs
3 Polymerase catalyzes
5-
phosphodiester linkage
3'
5'
3'
of two initial rNTPs.
5'
Nascent RNA
ELONGATION
4 Polymerase advances
5'
3-5' down template MamNMn
3'
strand, melting duplex
DNA and adding rNTPs
to growing RNA.
DNA-RNA
hybrid region
TERMINATION
5'
5'
MMの
3'
5 At transcription stop site,
polymerase releases
completed RNA and
dissociates from DNA.
3'
5'
Completed
RNA strand
Lodish et al., Molecular Cell Biology, 9e, © 2021 W. H. Freeman and Company
Transcribed Image Text:Stop site on template strand RNA polymerase Start site on template strand INITIATION 1 Polymerase binds to promoter sequence in duplex DNA. "Closed complex" 3' Promoter 2 Polymerase melts duplex DNA near transcription start site, forming a transcription bubble. 5- 3' Transcription bubble "Open complex" Initial rNTPs 3 Polymerase catalyzes 5- phosphodiester linkage 3' 5' 3' of two initial rNTPs. 5' Nascent RNA ELONGATION 4 Polymerase advances 5' 3-5' down template MamNMn 3' strand, melting duplex DNA and adding rNTPs to growing RNA. DNA-RNA hybrid region TERMINATION 5' 5' MMの 3' 5 At transcription stop site, polymerase releases completed RNA and dissociates from DNA. 3' 5' Completed RNA strand Lodish et al., Molecular Cell Biology, 9e, © 2021 W. H. Freeman and Company
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