Go to the NCBI’s website at https://ncbi.nlm.nih.gov On the database dropdown menu, select “Gene” and search for “RB1.” The first entry should be on the Homo sapiens version; click the gene name. Use the information to answer the following: Return to the RefSeq section in the Gene Database for RB1 (back click once from where you were for part c). Click on the link under “mRNA and Protein(s)” listed as NM_000321.3. This will take you to the mature mRNA sequence data: a. How many bases long is the full-length RB1 mRNA transcript? b. Scroll down to “Features.” Click on “CDS.” This will highlight the coding sequence region of the RB1 mRNA. This is the sequence that will be translated into a protein. At what positions (numbers) does the coding sequence start and stop? How long is the coding sequence? How many amino acids should this encode for? (HINT: does the stop codon encode an amino acid?)
Go to the NCBI’s website at https://ncbi.nlm.nih.gov On the database dropdown menu, select “Gene” and search for “RB1.” The first entry should be on the Homo sapiens version; click the gene name. Use the information to answer the following:
Return to the RefSeq section in the Gene Database for RB1 (back click once from where you were for part c). Click on the link under “mRNA and Protein(s)” listed as NM_000321.3. This will take you to the mature mRNA sequence data:
a. How many bases long is the full-length RB1 mRNA transcript?
b. Scroll down to “Features.” Click on “CDS.” This will highlight the coding sequence region of the RB1 mRNA. This is the sequence that will be translated into a protein.
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- At what positions (numbers) does the coding sequence start and stop?
- How long is the coding sequence?
- How many amino acids should this encode for? (HINT: does the stop codon encode an amino acid?)
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