EXPERIMENT 3: Why do enzymes react differently?DATA SHEETGiven the following standards and the corresponding standard curve table, what is theamount of substrate consumed given that 1.6 mM of substrate is prepared with anenzyme gave an absorbance of 0.345?0.40.81.21.6Substrate with enzymeConcentration (mM)Standard curve table:Varying Absorbance Absorbance AbsorbanceFinalInitialAcPComponentChangeDepletedumolmM540 nm540nm0.10.0370.0880.0290.07840.20.0740.0220.0520.14060.40.0740.0740.0860.1480.20000.60.2220.1360.23250.80.2960.2040.0920.24871.20.4440.3450.0990.26760.1040.1061.60.5920.7400.4880.28112.00.6340.2865

The enzymes are protein molecules that increase the rate of reactions by decreasing the activation…

Answered: Given the following standards and the…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Concentrations in biochemical systems are often very dilute. Consequently, scientific notation and logarithms are often used to express concentrations. In scientific notation, numbers are expressed as coefficient x 10" To convert a number to scientific notation, proceed as follows: 1. Move the decimal place so that there is one digit in front of the decimal. 2. Account for the moved decimal in the value of x. If the decimal moved to the right, x is negative; if it moved to the left, x is positive. A logarithm is basically an exponent. Unless otherwise indicated, a logarithm is the a of 10". The numbers after the decimal point are significant; the number before the decimal just identify the location of the decimal point for the number. Notice that it is easy to estimate a logarithm from scientific notation; it's the exponent! Logarithms are commonly used to express the concentration of H. The pH is defined as pH= log (In), where the base number is 2.303. The same general rules as logs…
in food microbiology, how do you compute for concentration (M) and absorbance (A) of peroxidase activity on hydrogen peroxide at different pH values? (wavelength used = 415nm). can you please explain the calculations thank you if given: Temperature = 75 C Molar absorptivity coefficient = 10.5 Path length (cm) = 2 I = transmitted light = 0.45
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