Given the following standards and the corresponding standard curve table, what is the amount of substrate consumed given that 1.6 mM of substrate is prepared with an enzyme gave an absorbance of 0.345? 0.1 0.2 0.4 EXPERIMENT 3: Why do enzymes react differently? DATA SHEET 0.6 0.8 1.2 1.6 2.0 0.4 0.8 1.2 1.6 Standard curve table: Varying Absorbance Absorbance Absorbance Component Initial Final mM 540 nm 540nm Concentration (mm) 0.037 0.074 0.148 0.222 0.296 0.444 0.592 0.740 0.008 0.022 0.074 0.136 0.204 0.345 0.488 0.634 AcP Change Depleted μmol 0.029 0.052 0.074 0.086 0.092 0.099 0.104 0.106 0.0784 0.1406 0.2000 0.2325 0.2487 0.2676 0.2811 0.2865 Substrate with enzyme
Given the following standards and the corresponding standard curve table, what is the amount of substrate consumed given that 1.6 mM of substrate is prepared with an enzyme gave an absorbance of 0.345? 0.1 0.2 0.4 EXPERIMENT 3: Why do enzymes react differently? DATA SHEET 0.6 0.8 1.2 1.6 2.0 0.4 0.8 1.2 1.6 Standard curve table: Varying Absorbance Absorbance Absorbance Component Initial Final mM 540 nm 540nm Concentration (mm) 0.037 0.074 0.148 0.222 0.296 0.444 0.592 0.740 0.008 0.022 0.074 0.136 0.204 0.345 0.488 0.634 AcP Change Depleted μmol 0.029 0.052 0.074 0.086 0.092 0.099 0.104 0.106 0.0784 0.1406 0.2000 0.2325 0.2487 0.2676 0.2811 0.2865 Substrate with enzyme
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Transcribed Image Text:EXPERIMENT 3: Why do enzymes react differently?
DATA SHEET
Given the following standards and the corresponding standard curve table, what is the
amount of substrate consumed given that 1.6 mM of substrate is prepared with an
enzyme gave an absorbance of 0.345?
0.4
0.8
1.2
1.6
Substrate with enzyme
Concentration (mM)
Standard curve table:
Varying Absorbance Absorbance Absorbance
Final
Initial
AcP
Component
Change
Depleted
umol
mM
540 nm
540nm
0.1
0.037
0.088
0.029
0.0784
0.2
0.074
0.022
0.052
0.1406
0.4
0.074
0.074
0.086
0.148
0.2000
0.6
0.222
0.136
0.2325
0.8
0.296
0.204
0.092
0.2487
1.2
0.444
0.345
0.099
0.2676
0.104
0.106
1.6
0.592
0.740
0.488
0.2811
2.0
0.634
0.2865
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