A 455.0-mg sample of imported meat product was analyzed for its protein content. A volume of 12.59 mL of 0.2250 M HCI was required to titrate the liberated ammonia.
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- Given the following information, calculate the total activity in the undiluted protein sample. Activity of 1 ml of diluted sample = 0.5 Total volume of sample = 5 ml Dilution factor = 10 25 50.5 250 2.5What rate in mL/hr of Isosource VHN would provide 1.6 g protein/kg/day if run for 24 hours for a patient weighing 68 kg? Round to the nearest whole number, do not enter units EN Product Кcal Protein /mL g/L General Formulations Jevity 1.5 1.5 63.4 Isosource VHN 1 62 TwoCal HN 2 83 Renal Formulations Nepro 1.8 81 Renalcal 2 34.4 Supplena 1.8 45 Glucose Control Formulations Glucerna 1 41 Choice DM 1 45You have just used up the last of a 1.2 % m/m solution of agarose in water. You require another 100mls. How many grams of Agarose do you require? Note 1ml of water=1g What is the % w/v of a 100ml solution containing 5g of NaCl?
- 1.2461 g of a wheat sample were analyzed to find out the protein content, by the method Kjeldahl, with an excess of H3BO3 in the distillate receiving vessel. Calculate the% nitrogen if 13.19 mL of 0.0962 M HCl were needed to reach the bromocresol green end point. (Pat N, 14.0)What is the amount of dextrose in mg/kg/min provided by the following TPN for a 60 kg patient? Round to 1 decimal place, do not enter units Dextrose (70%) 320 g Amino acids (8.5%) 92 g Lipids (10%) 44 g sodium chloride 60 mEq sodium acetate 40 mEq Sodium phosphate 15 mmol potassium chloride 80 mEq potassium acetate 30 mEq calcium gluconate 18 mEq magnesium sulfate 8 mEq Multivitamins 10 mL Trace elements 1 mL Total volume: 2640 mL Rate: 110 mL/hrA mixture of three proteins was subjected to ion exchange chromatography using CM-cellulsoe as the stationary phase. The elution buffer had a pH of 7.5. Proteins were eluted by increasing the ionic strength of the buffer. Predict the order of elution of the following proteins:Pepsinogen, pI = 1Cytochrome c, pI = 10.6Myoglobin, pI = 6.8
- You need a buff er at pH 7.5 for use in purifying a protein at 4°C. You have chosen Tris, pK 8.08, ΔH° = 50 kJ · mol−1. You carefully make up 0.01 M Tris buffer, pH 7.5 at 25°C, and store it in the cold to equilibrate it to the temperature of the purifi cation. When you measure the pH of the temperature-equilibrated buff er it has increased to 8.1. What is the explanation for this increase? How can you avoid this problem?Using the data from the standard curve done previously, you record the absorbance of your extracted milk protein sample at 0.499 at 540nm. What is the mass of the protein in mg assuming you collected 10mL worth of the protein? Your standard curve should have a line of best fit with the equation y=0.0001x+0.0038.Quinine ( C20 H24 N2 O2) is the most important alkaloid derived from cinchona bark. It is used as an antimalarial drug. For quinine, pK, = 5.1 and pK, = 9.7 ( pKp = – log Kp). Only 1 g quinine will dissolve in 1920.0 mL of solution. Calculate the pH of a saturated aqueous solution of quinine. Consider only the reaction | Q+ H2O= QH+ + OH- described by pK, where Q = quinine. pH =
- How many mL of the 1 M glucose stock solution do you need to prepare 100 mL of a 1 mM glucose solution?The glucose content of samples can be determined using Nelson't test. The equation of the line for the standard curve preapred for varying concentrations of glucose is y = 3.0x + 0.2. Based from this information, determine the glucose concentration (in M) of a strawberry preserve having an absorbance reading of 1.35.Paclitaxel is used to treat ovarian cancer by infusion at a concentration of 0.6 mg/mL over 24 hours. A dose of 135 mg/m² has been prescribed for Sero. Sero is 1.06 m tall and weighs 92 kg. Paclitaxel comes in vials of 150 mg/25 mL. Calculate the volume of 5%w/v glucose solution that would be required to dilute the concentrated solution to prepare an paclitaxel solution of the required strength.