Determine the CFU/mL of the pasteurized milk given the following bacterial counts obtained from raw milk and pasteurized milk: (Vp = 1 mL). Show your solution and express your final answer in 2 significant figures. Dilution Raw Milk Pasteurized Milk 486, 397 224, 218 102, 97 2,0 1:10 32, 29 0,0 0,0 0,0 1:100 1:1000 1:10 000

Q: The solution containing bacterial spores is heated in an autoclave. When the autoclave has reached a…

A: Given that          Number of spores after autoclave reaches 121 C (No) = 106 spores/ml…

Q: 14 : 127 OPFective mae Lyrol Amm Bleach :.81 CPpective amm 10 ::90 moe Lirterine lomm ppective

A: *disinfectants are used to control  infections by killing microorganisms. *the disinfectants will…

Q: What is the minimal concentration (MIC) for disinfectant number 1? Just write the corresponding…

A: Minimum Inhibitory Concentration is defined as the least concentration of a substance that does not…

Q: A culture is incubated for 10 hours. 1 hours after inoculation it reached the exponential growth…

A: This question involves the calculation of the growth constant (k) and generation time (g(t)) for a…

Q: 10ul of the original sample was transferred to 90ul diluent. Then, the mixture was mixed with 100ul…

A: Hemocytometer is blood cell counting device. It is usally cposed of thick glass on which square…

Q: Calculate the CFU present in 150 mL water sample and b) dilution factor up to 5. Consider the…

A: The serial dilution process is used to calculate the number of microorganisms present in a…

Q: This is the growth curve for Clostirdium (incubated under optimal growth conditions). Use it to…

A: There are 5 phases to a logistic (sigmoidal) growth curve.  They are stationary phase, lag phase,…

Q: how many ml of sterile water injection must be mixed with two 4 ml vials of sterile hypertonic NaCl…

A: Tonicity refers to the ability of a solvent to cause cells to shrink or swell. A semipermeable…

Q: Silver mat 13 mm : 1:18 eppecetive Nitrate Aqueous lodine 22 moro 2 mm 2 Oppective

A: Disinfectant: An agent or compound that kills the bacteria or makes it functionally inactive. There…

Q: Calculate the coliform concentration in a milk sample based on the following colony counts: 10 2 :…

A: Introduction Coliform bacteria are defined as rod-shaped Gram-negative non-spore forming and motile…

Q: 4 mL of 10% TCA solution was added to 1 mL of serum and after mixing, it was waited for two minutes…

A: Calibration graphs are used to estimate the concentration of unknown sample by plotting it against…

Q: Dilution: 10-

A: intoduction   A Serial weakening is a progression of weakenings, with the weakening variable…

Q: As a public health engieer you have been tasked wiyh designing a plug flow reactor for the…

A: The Surface Water Treatment Rule, or SWTR for short, is an EPA regulation that establishes drinking…

Q: The number of bacterial cells in a culture broth is to be determined by a culture technique. Serial…

A: Colony forming unit refers to the measurement of the number of viable cells present in per ml of the…

Q: Determine the minimum inhibitory concentration and the minimum exposure time given the following…

A: MIC is the lowest concentration where we can observe the visible decline in the bacterial population…

Q: Solution Stock solution Working solution Volume needed restriction digestion buffer 10X DNA 200…

A: In order to set up restriction digestion, we must mix precise amounts of various components to get…

Q: In using the centrifuge machine, how will this be remedied if there are only 3 separate liquid…

A: Centrifuges are laboratory equipment that is used to separate components in a mixture based on their…

Q: You have a 1:5 dilution. Describe how you would make a 1:100 dilution.  When 0.5 µL of serum is…

A: DF=Volume of final diluted solutionVolume removed from stock and added to new dilution   Dilution…

Q: #8, 9, 10 mode of action only

A: Disinfection is the process of eliminating germs and microbes from inanimate surfaces. Disinfection…

Q: Order: Zosyn 0.02 gm/kg Supply: 5 gm powder vial with directions to add 10 mL of sterile water as…

A: Here we have to calculate how much diluent we need to use to reconstitute Zosyn.

Q: chemical(s) best at inhibiting both Gram- negative and Gram-positive bacteria [Choose ] [Choose ]…

A: Hi! Thanks for your question. As you have posted multiple questions and have not mentioned which one…

Q: If the 10-9 plate was counted to have 155 colonies on it, would the CFU/mL be 1.55*1011? If not,…

A: CFU  Colony forming unit it a method to calculate the number of microorganisms in a culture media.

Q: Compute for the bacteria/ml of raw milk at dilution factor of 100, if bacterial cell counts in 10…

A: Bacteria  Sub microscopic organisms that are found almost every where on earth. They may be useful…

Q: solve for the cfu/ml in a given of 300 colonies at plate 10^4. show complete solution. see attached…

A: CFU or colony forming unit is the viable count of bacterial/fungal cells. In direct microscopic…

Q: You are asked to make 10 mL of buffer from a 5X concentrate. invitrogen Te rerder visit…

A: In their working range, buffers resist change in pH. Buffers consist of a weak acid and its…

Q: a. Let’s say for example that a milk sample has 10,000 bacteria per milliliters. If 1 mL of this…

A: A serial dilution is a process of sequential dilution in order to decrease the density of cell…

Q: A bacterial culture has a concentration of 3.2 x 108 cells /mL.  You dilute this culture as follows:…

A: CFUs are a measure of the number of progenitors present in a given population of cells; if an…

Q: O 5 P.A S.A. 2.

A: In this method paper disc saturated with a specific anti microbial agent one at a time is placed on…

Q: Indicate whether you think that the number of cfu/ ml calculated would be too high or too low if you…

A: Cfu refers to the colony forming unit which is formed through continuous replication of a single…

Q: cm-1 4000.0 3000 2000 1500 1000 400.0 0.0 3326.35- 316230 1654.89- 10 1506.31 1441.14- 1258.93…

A: Mass spectroscopy and IR spectroscopy are methods of spectroscopy which is used for the…

Q: A milk sample was tested for the presence of typhimurium. Using a 10 microliter calibrated loop, a…

A: Serial dilution is the technique to find the concentration of bacteria in a sample when there are…

Q: Using 10 μL of a patient's serum, how much diluent would be needed to create a 1:10 dilution? ______…

A: Dilution is the technique of lowering the concentration of a solute in a solution by merely adding…

Q: a physician prescribed an antibiotic to be mixed in white petrolatum to produce a 25% antibiotic…

A: Ointment preparations with antibiotics in petrolatum are useful for local external…

Q: mifes Time CFU at 48 hours Data Table 3 Plate description Dilution: 10-1 Incubation time: 24…

A: CFU is a measure of total microbial count (bacterial) that can be found in the given testing…

Q: 50 g of food is homogenized in 450 mL of water. From this, 1 mL is added to 29 mL of water, and then…

A: Whenever a microrganism is plated for analytical purposes, it population is reported in terms of…

Q: Where do you expect to see the highest number of CFU on a plate: Select one: a. When pasteurized…

A: The number of colony forming units on the countable plate is multiplied by 1/FDF to get the number…

Q: 5. Provided with the following data, compute the corresponding CFU/ml of the original culture.…

A: Answer :: Introduction The plate is considered to be countable if it has a colony count between 30…

Q: OCULAR ОBJECTIVE TOTAL MAGNIFICATION 10x 1. 100x 10х 2. 400x 10х 100x 3. 10x 5x 4.

A: A microscope is an instrument which is generally used in laboratory to examine objects that are so…

Q: For the following problem indicate the values for each part of the equation and solve for the…

A: The process of lowering the concentration of a solution by adding solvent to it is called dilution.…

Q: o NB4: Why do you think you first had to make the 1 mM solution instead of working directly from the…

A: A working solution is different from Stock solution. A Working solution is prepared from stock…

Q: (b) A Food material containing Bacillus stearothermophilus PS1518 as an indicator organism ts…

A: Given : F0 = integration t0[(T-T0)/z]dt

Q: The number of bacterial cells in a culture broth is to be determined by a culture technique. Serial…

A: Serial dilution is the reduction of micro-organisms through successive dilutions. By serial…

Q: A cell count was performed on a serous fluid specimen.  As the fluid was cloudy, a 1:5 dilution was…

A: The subject is a cell count from a sample of serous fluid that was previously described as hazy. A…