Explain the role of boundary elements in the DNA. If you were to use gene editing technology to remove the IR-L boundary element, what would happen to the chromatin structure in this area? Why? Euchromatin mat1 Euchromatin Boundary Boundary Heterochromatin 2.5 kb IR-L mat2P mat3M IR-R cenH 66...
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- Let’s suppose you have isolated chromatin from some bizarreeukaryote with a linker region that is usually 300–350 bp inlength. The nucleosome structure is the same as in other eukaryotes.If you digested this eukaryotic organism’s chromatin with ahigh concentration of DNase I, what would be your expectedresults?Forming nucleosomes and wrapping them into a 30-nm fiber provide part of the compaction of DNA in chromatin. If the fiber contains about six nucleosomes per 10 nm of length, what is the approximate compaction ratio achieved?several options can be correct Consider the following segment of DNA, which is part of a linear chromosome: LEFT 5’.…TGACTGACAGTC….3’ 3’.…ACTGACTGTCAG….5’ RIGHT During RNA transcription, this double-strand molecule is separated into two single strands from the right to the left and the RNA polymerase is also moving from the right to the left of the segment. Please select all the peptide sequence(s) that could be produced from the mRNA transcribed from this segment of DNA. (Hint: you need to use the genetic codon table to translate the determined mRNA sequence into peptide. Please be reminded that there are more than one reading frames.) Question 6 options: ...-Asp-Cys-Gln-Ser-... ...-Leu-Thr-Val-... ...-Thr-Val-Ser-... ...-Leu-Ser-Val-... ...-Met-Asp-Cys-Gln-...
- Compared to heterochromatin, euchromatin is comprised of densely packed nucleosomes and with higher transcription activity comprised of densely packed nucleosomes and with lower transcription activity O comprised of loosely packed nucleosomes and with lower transcription activity Ocomprised of loosely packed nucleosomes and with higher transcription activityGiven the coding strand DNA sequence below, what change would occur in the expressed protein sequence if a mutation resulted in insertion of C between the final two nucleotides? 5'-AATGCCGTAA-3' Expressed protein would be extended (lengthened) O Correct answer not given No change would occur in the expressed pratein OExpressed protein would be truncated (shortened) OA single amino acid in the expressed protein would be substitutedwhat are we looking at in part (b)? Is this an11-nm fiber, a 30-nm fiber, or a 300-nm fiber? Does this DNAcome from a cell during M phase or interphase?
- A part of a sequenced chromosome has the sequence (on one strand)ATTGCATCCGCGCGTGCGCGCGCGATCCCGTTACTTTCCG. Which part of thissequence is most likely to take up the Z conformation?Assuming that the 30-nm chromatin fiber con-tains about 20 nucleosomes (200 bp/nucleosome) per 50nm of length, calculate the degree of compaction of DNAassociated with this level of chromatin structure. Whatfraction of the 10,000-fold condensation that occurs atmitosis does this level of DNA packing represent?The picture below depicts electron micrographs of the major levels of chromatin structure. Match each of the listed conditions with the most likely levels of chromatin structure under that condition.
- What size DNA fragment would be released after very mild digestion of chromatin with micrococcal nuclease? (This would represent a single cut per nucleosome.) 30 bp O 165 bp 146 bp 200 bpLet’s suppose you have isolated chromatin from some bizarre eukaryote that has a DNA linker region that is usually 300 to 350 bp in length. The nucleosome structure is the same as in other eukaryotes. If you digested this eukaryotic organism’s chromatin with a high concentration of DNase I, what would be your expected results?4b) What is the functional difference between heterochromatin and euchromatin? In other words, what can a cell do (or not do) with euchromatic DNA but not heterochromatic DNA?