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Double-stranded CDNA is synthesized from mRNA Intron Exon Intron Transcription (in cell) Introns removed, polyA tail added (in cell) AAAA Oligo-dT primer anneals to polyA tail Reverse transcriptase copies MRNA into CDNA MRNA Single-stranded CDNA MRNA removed DNA polymerase copies CDNA strand Nick hairpin 3' Double-stranded 5' CDNA FIGURE 10-4 The formation of CDNA for the insulin gene. The insulin gene (with its two introns) is transcribed in the pancreas into pre-MRNA. The introns are removed by splicing, and A residues are added to the 3' end to form polyadenylated MRNA. In the laboratory, MRNAS are isolated from pancreatic cells and a short oligo-dT primer is hybridized to the poly(A) tail of all MRNAS to prime synthesis of complementary DNA from the RNA template by reverse transcriptase. Reverse transcriptase synthesizes a stem-loop structure that acts as a primer for synthesis of the second CDNA strand, after the MRNA strand has been degraded (by treatment with NaOH or with RNASEH). io in

Double-stranded CDNA is synthesized from mRNA Intron Exon Intron Transcription (in cell) Introns removed, polyA tail added (in cell) AAAA Oligo-dT primer anneals to polyA tail Reverse transcriptase copies MRNA into CDNA MRNA Single-stranded CDNA MRNA removed DNA polymerase copies CDNA strand Nick hairpin 3' Double-stranded 5' CDNA FIGURE 10-4 The formation of CDNA for the insulin gene. The insulin gene (with its two introns) is transcribed in the pancreas into pre-MRNA. The introns are removed by splicing, and A residues are added to the 3' end to form polyadenylated MRNA. In the laboratory, MRNAS are isolated from pancreatic cells and a short oligo-dT primer is hybridized to the poly(A) tail of all MRNAS to prime synthesis of complementary DNA from the RNA template by reverse transcriptase. Reverse transcriptase synthesizes a stem-loop structure that acts as a primer for synthesis of the second CDNA strand, after the MRNA strand has been degraded (by treatment with NaOH or with RNASEH). io in

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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In Figure 10-4, why is cDNA made only from mRNA
and not also from tRNAs and ribosomal RNAs

Double-stranded CDNA is synthesized from mRNA Intron Exon Intron Transcription (in cell) Introns removed, polyA tail added (in cell) AAAA Oligo-dT primer anneals to polyA tail Reverse transcriptase copies MRNA into CDNA MRNA Single-stranded CDNA MRNA removed DNA polymerase copies CDNA strand Nick hairpin 3' Double-stranded 5' CDNA FIGURE 10-4 The formation of CDNA for the insulin gene. The insulin gene (with its two introns) is transcribed in the pancreas into pre-MRNA. The introns are removed by splicing, and A residues are added to the 3' end to form polyadenylated MRNA. In the laboratory, MRNAS are isolated from pancreatic cells and a short oligo-dT primer is hybridized to the poly(A) tail of all MRNAS to prime synthesis of complementary DNA from the RNA template by reverse transcriptase. Reverse transcriptase synthesizes a stem-loop structure that acts as a primer for synthesis of the second CDNA strand, after the MRNA strand has been degraded (by treatment with NaOH or with RNASEH). io in
Transcribed Image Text:Double-stranded CDNA is synthesized from mRNA Intron Exon Intron Transcription (in cell) Introns removed, polyA tail added (in cell) AAAA Oligo-dT primer anneals to polyA tail Reverse transcriptase copies MRNA into CDNA MRNA Single-stranded CDNA MRNA removed DNA polymerase copies CDNA strand Nick hairpin 3' Double-stranded 5' CDNA FIGURE 10-4 The formation of CDNA for the insulin gene. The insulin gene (with its two introns) is transcribed in the pancreas into pre-MRNA. The introns are removed by splicing, and A residues are added to the 3' end to form polyadenylated MRNA. In the laboratory, MRNAS are isolated from pancreatic cells and a short oligo-dT primer is hybridized to the poly(A) tail of all MRNAS to prime synthesis of complementary DNA from the RNA template by reverse transcriptase. Reverse transcriptase synthesizes a stem-loop structure that acts as a primer for synthesis of the second CDNA strand, after the MRNA strand has been degraded (by treatment with NaOH or with RNASEH). io in
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