DNA isolated from the bacterial virus M13 con-tains 25% A, 33% T, 22% C, and 20% G. Do these resultsstrike you as peculiar? Why or why not? How might youexplain these values?

Q: the TPOX allele with the structure [TAGA]10 will be ______ nucleotides longer than the allele with…

A: [TAGA]10  will have four nucleotides longer than the allele with the structure [TAGA]5

Q: Calculate the concentration of plasmid DNA if OD260 value is 0.74 and OD280 value is 0.35 (taken…

A: Introduction A plasmid is a short extrachromosomal DNA molecule that can replicate independently of…

Q: There was a mix of virus and E.coli DNA thats isolated and separated .  One DNA sample  had a base…

A: DNA (deoxyribonucleic acid) was discovered by Friedrich Miescher. Nucleotides are the structural…

Q: By conducting conjugation experiments between Hfr and recipientstrains, Wollman and Jacob mapped the…

A: The interrupted mating technique is a technique used to map the genes in the chromosome of the…

Q: In the name E. coli 4X5B, which part is called the serotype, serovar, or strain?     E     coli…

A: A microbe is a living entity that is so tiny that it cannot be seen with the naked eye. Microbiology…

Q: What is the name of the process in which a single strand of DNA disrupts a homologous dsDNA molecule…

A: primers the short, single-stranded nucleotide sequence that is used in the DNA synthesis process.…

Q: The letter R isconventionally used to represent anypurine base (A or G) and Y to represent…

A: Purines and pyrimidines are the two families of nitrogeneous base that are used to make nucleic…

Q: Why is DNA supercoiling called supercoiling rather than just coiling? Why is positive supercoiling…

A: DNA supercoiling is an important process for the cells as it allows the packings of large DNA into a…

Q: pXO1 and pXO2 are large plasmids – large pieces of DNA that are not the chromosome that can…

A: Plasmids are the extra chromosomal material which are present independently of  the main…

Q: Norflaxacin is a cidal broad spectrum antibiotic that functions by inhibiting DNA gyrase in gram +/-…

A: Norfloxacin is a broad-spectrum fluoroquinolone antibiotic, which is used to treat UTI (urinary…

Q: molecules continues to double every temperature cycle (referred to as a PCR cycle). (a) Suppose a…

A: Note: We can only answer one question at a time so please resubmit other questions one by one.…

Q: Why does the DNA replication happen in the 5`-3` direction instead of the 3`-5`

A: DNA replication is the biological process by which DNA synthesis two identical replicas of itself…

Q: A commercially available strain of P. syringae marketed as FrostbanB is used to combat frost damage.…

A: Recombination is a process of exchanging genetic material between different organisms. This process…

Q: Why is the presence of insertion sequences on the F plasmid significant? What are F- prime (or F')…

A: The process of fusion of two compatible bacterial cells, is known as Conjugation. It requires the…

Q: Why is DNA pol III still a thing, given that it lacks some of the functionality of DNA pol I - why…

A: The Central Dogma concept is a very important part of Molecular Biology. It states that "DNA makes…

Q: This virus does not conform to the central dogma of molecular biology. Explain the central dogma and…

A: In this discussion, we'll investigate the complexities of certain infections that resist the central…

Q: What do you mean by bacteriophage?

A: Viruses can affect only specific species of hosts and only particular cells in that host. The virus…

Q: Bacteriophage T7 helicase movesalong DNA in the 5' -to-3' direction.Other helicases have been…

A: it is a DNAB protein it is a hexamer of identical subunit they clamp around each of the two single…

Q: How many bacteriophage particles were isolated from a single plaque? Howmany different strains of…

A: Bacteriophages, which are mainly infected bacteria are going through the lytic or the lysogenic…

Q: Wild-type E. coli takes up and concentrates a certain redfood dye, making the colonies blood red.…

A: As it is given that Wild-type E. coli concentrate the certain red dye by taking up it and thus make…

Q: Price et al. [(1999). J. Bacteriol. 181:2358–2362] conducteda genetic study of the toxin transport…

A: There are basically two main types of gene transfer - Vertical gene transfer and Horizontal gene…

Q: A bacterium has a genome size of 4.4 Mb. If a researchercarries out shotgun DNA sequencing and…

A: Shotgun sequencing is a technique for determining the sequence of entire chromosomes and entire…

Q: Price et al. [(1999). J. Bacteriol. 181:2358–2362] conducteda genetic study of the toxin transport…

A: Genes are the functional units of DNA and express a functional protein. They are made up of…

Q: All are propertles of nucleosomes EXCEPT: / Almal is eienskappe van nukleosome, behalwe: A. all are…

A: Nucleosome They are fundamental repeating subunits of all eukaryotic chromatin (except when…

Q: A segment of DNA from theinterior of a single strand is shown inFigure Q4–1. What is the polarity of…

A: DNA, is a nucleic acid it is deoxyribonucleotide, it is called deoxy because the 2 carbon position…

Q: An Hfr strain that is hisE+ and pheA+ was mixed with a strain thatis hisE− and pheA−. The…

A: Transfer of genes in prokaryotes occurs through horizontal transfer mechanisms such as transduction,…

Q: How many nanograms of DNA should we expect to be contained within the slowest band from the…

A: pGLO plasmid: pGLO is a plasmid vector mostly used for the expression of genes.  The pGLO plasmid…

Q: IT has been shown that when a thin beam of light is aimedat a nucleus, the amount of light absorbed…

A: Heredity as we know is the passing on of traits from parents onto their offspring. Organisms that…

Q: In the “How we know” regarding the origin of replication and cell survival on ampicillin, why does…

A: Without an origin of replication , replication cannot occur.

Q: Unknown virus dsDNA contains 36% AT on a molar basis. What are the mole percentages of A, G and C…

A: Double-stranded (ds) DNA (Deoxyribonucleic acid) is composed of two strands. DNA is composed of…

Q: Given the following ssDNA, what would be the complementary strand for it to be dsDNA?…

A: Both strands of DNA are complementary to each other. Adnan bond with thymine via double bond however…

Q: DNA containing the genes gin+ phe+ lys+ was used to transform bacterial cells of genotype gln- phe-…

A: As per the question :-  Genotypes and number of individuals obtained are as:-  A ) gly + phe+ lys +…

Q: Because bacteria are able to replicate at high rates, they aresubjected to mistakes or mutations…

A: Mutations in genes Single DNA base alterations, as well as minor intragenic deletions and…

Q: DNA is isolated from concentrated ocean water. One genome is studied, and the proportion of each…

A: DNA is made of of pentose sugar, nitrogenous base, phosphate group which forms a nucleotide and each…

Q: Researchers who study the molecular mechanism of transformationhave identified many proteins in…

A: DNA is the genetic material in most living organisms. It is the information hub of the cell that…

Q: Homologous recombination in E. coli forms heteroduplex regions of DNA containing mismatched bases.…

A: MMR of Mismatch Repair system of DNA is referred to as a system for identifying and repairing the…

Q: The sequence shown in Figure 2 is the nucleotide sequence of a heat shock protein of Bacillus…

A: For the purpose of figuring out a gene's or protein's properties and function, nucleotide sequence…

Q: Following formation of a double-stranded break, enzymes process the dsDNA end to form…

A: DNA end resection is a key process in the cellular response to DNA double-strand break damage that…

Q: E. coli cells are simultaneously infected with two strains of phage λ. One strain has a mutant host…

A: A gene is a stretch of nucleotides present in the DNA molecule. It encodes information for the…

Q: In Figure 7-7, do you recognize any of the componentsused to make Watson and Crick’s DNA model?…

A: DNA is made up of nucleotidea. Each nucleotide is made up of a phosphate group, dexy ribose pentose…

Viral Replication

The virus is acellular and considered non-living outside the host cell. The replication of the virus occurs only inside the living cells. Only a few genes are present in the smallest virus, whereas many genes are present in the largest virus. These genes encode specific viral proteins.