Cyt cb562 will form a tetramer in the presence of Zn+2 or in the absence of Zn+2, it will form a trimer. For both the tetramer and the trimer, suggest the identity of three different amino acids that could be on the interface. Explain how your selections facilitate these interactions.
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Cyt cb562 will form a tetramer in the presence of Zn+2 or in the absence of Zn+2, it will form a trimer. For both the tetramer and the trimer, suggest the identity of three different amino acids that could be on the interface. Explain how your selections facilitate these interactions.
INTRODUCTION :
Cyt cb562 - Cytochrome cb562 is a variant of an Escherichia coli four-helix bundle b-type heme protein in which the porphyrin prosthetic group is covalently ligated to the polypeptide near the terminus of helix 4.
The four-helix-bundle protein fold can be constructed from a variety of primary amino acid sequences. Proteins with this structure are excellent candidates for investigations of the relationship between folding mechanism and topology. The folding of cytochrome b(562), a four-helix-bundle heme protein, is hampered by heme dissociation. To overcome this complication, we have engineered a variant of cyt b(562)which is (cyt c-b(562)) featuring a c-type linkage between the heme and the polypeptide chain.
Tetramer & Trimer -
Tetramer - A tetramer is an oligomer formed from four monomers or subunits. The associated property is called tetramery. An example from inorganic chemistry is titanium methoxide with the empirical formula Ti(OCH₃)₄, which is tetrameric in solid state and has the molecular formula Ti₄(OCH₃)₁₆ .
Trimer - A trimer is a molecule or polyatomic anion formed by combination or association of three molecules or ions of the same substance. In technical terms, a trimer is a kind of oligomer derived from three identical precursors often in competition with polymerization.
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