Amino acid analysis of a HEPTAPEPTIDE reveals the following information below: (NOTE: when the sequence is not known, a comma separates the amino acids) R and modified free amino acids Peptide 1 (K, R, V) Hydrazine Peptide 2 (K, M, W, Y) HEPTAPEPTIDE Cleavage with Cyanogen bromide, then chromatography 6N HCL, 100 °C, 24 h Phenylisothiocyanate Cleavage with Chymotrypsin, then chromatography Cleavage with Thermolysin, then chromatography K, K, M, R, V, W, Y PTH derivative of Y Peptide 3 (K, M, R, V) Peptide 4 (K, W) Free Y Peptide 5 (K, Y) Peptide 6 (R, V) Peptide 7 (K, M, W)
Amino acid analysis of a HEPTAPEPTIDE reveals the following information below: (NOTE: when the sequence is not known, a comma separates the amino acids) R and modified free amino acids Peptide 1 (K, R, V) Hydrazine Peptide 2 (K, M, W, Y) HEPTAPEPTIDE Cleavage with Cyanogen bromide, then chromatography 6N HCL, 100 °C, 24 h Phenylisothiocyanate Cleavage with Chymotrypsin, then chromatography Cleavage with Thermolysin, then chromatography K, K, M, R, V, W, Y PTH derivative of Y Peptide 3 (K, M, R, V) Peptide 4 (K, W) Free Y Peptide 5 (K, Y) Peptide 6 (R, V) Peptide 7 (K, M, W)
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
asap pls

Transcribed Image Text:Amino acid analysis of a HEPTAPEPTIDE reveals the following information below:
(NOTE: when the sequence is not known, a comma separates the amino acids)
R and modified
free amino acids
Peptide 1
(K, R, V)
Hydrazine
Peptide 2
(K, M, W, Y)
HEPTAPEPTIDE
Cleavage with
Cyanogen
bromide, then
chromatography
1. What is the N-terminal residue?
Cleavage with Chymotrypsin,
then chromatography
Cleavage with Thermolysin,
then chromatography
2. What is the sequence of Peptide 1?
6N HCL, 100 °C, 24 h
Phenylisothiocyanate
3. What is the sequence of Peptide 3?
4. What is the sequence of Peptide 5?
When answering the questions below, please use the ONE-LETTER CODE (UPPERCASE) for the amino acid, with NO spaces and symbols between each letter.
5. What is the overall amino acid sequence?
K, K, M, R, V, W, Y
PTH derivative of Y
Peptide 3 (K, M, R, V)
Peptide 4 (K, W)
Free Y
Peptide 5 (K, Y)
Peptide 6 (R, V)
Peptide 7 (K, M, W)
Expert Solution

This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
This is a popular solution!
Trending now
This is a popular solution!
Step by step
Solved in 2 steps

Recommended textbooks for you

Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman

Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman

Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY

Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman

Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman

Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY

Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning

Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning

Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON