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Amino acid analysis of a HEPTAPEPTIDE reveals the following information below: (NOTE: when the sequence is not known, a comma separates the amino acids) R and modified free amino acids Peptide 1 (K, R, V) Hydrazine Peptide 2 (K, M, W, Y) HEPTAPEPTIDE Cleavage with Cyanogen bromide, then chromatography 6N HCL, 100 °C, 24 h Phenylisothiocyanate Cleavage with Chymotrypsin, then chromatography Cleavage with Thermolysin, then chromatography K, K, M, R, V, W, Y PTH derivative of Y Peptide 3 (K, M, R, V) Peptide 4 (K, W) Free Y Peptide 5 (K, Y) Peptide 6 (R, V) Peptide 7 (K, M, W)

Amino acid analysis of a HEPTAPEPTIDE reveals the following information below: (NOTE: when the sequence is not known, a comma separates the amino acids) R and modified free amino acids Peptide 1 (K, R, V) Hydrazine Peptide 2 (K, M, W, Y) HEPTAPEPTIDE Cleavage with Cyanogen bromide, then chromatography 6N HCL, 100 °C, 24 h Phenylisothiocyanate Cleavage with Chymotrypsin, then chromatography Cleavage with Thermolysin, then chromatography K, K, M, R, V, W, Y PTH derivative of Y Peptide 3 (K, M, R, V) Peptide 4 (K, W) Free Y Peptide 5 (K, Y) Peptide 6 (R, V) Peptide 7 (K, M, W)

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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Amino acid analysis of a HEPTAPEPTIDE reveals the following information below: (NOTE: when the sequence is not known, a comma separates the amino acids) R and modified free amino acids Peptide 1 (K, R, V) Hydrazine Peptide 2 (K, M, W, Y) HEPTAPEPTIDE Cleavage with Cyanogen bromide, then chromatography 1. What is the N-terminal residue? Cleavage with Chymotrypsin, then chromatography Cleavage with Thermolysin, then chromatography 2. What is the sequence of Peptide 1? 6N HCL, 100 °C, 24 h Phenylisothiocyanate 3. What is the sequence of Peptide 3? 4. What is the sequence of Peptide 5? When answering the questions below, please use the ONE-LETTER CODE (UPPERCASE) for the amino acid, with NO spaces and symbols between each letter. 5. What is the overall amino acid sequence? K, K, M, R, V, W, Y PTH derivative of Y Peptide 3 (K, M, R, V) Peptide 4 (K, W) Free Y Peptide 5 (K, Y) Peptide 6 (R, V) Peptide 7 (K, M, W)
Transcribed Image Text:Amino acid analysis of a HEPTAPEPTIDE reveals the following information below: (NOTE: when the sequence is not known, a comma separates the amino acids) R and modified free amino acids Peptide 1 (K, R, V) Hydrazine Peptide 2 (K, M, W, Y) HEPTAPEPTIDE Cleavage with Cyanogen bromide, then chromatography 1. What is the N-terminal residue? Cleavage with Chymotrypsin, then chromatography Cleavage with Thermolysin, then chromatography 2. What is the sequence of Peptide 1? 6N HCL, 100 °C, 24 h Phenylisothiocyanate 3. What is the sequence of Peptide 3? 4. What is the sequence of Peptide 5? When answering the questions below, please use the ONE-LETTER CODE (UPPERCASE) for the amino acid, with NO spaces and symbols between each letter. 5. What is the overall amino acid sequence? K, K, M, R, V, W, Y PTH derivative of Y Peptide 3 (K, M, R, V) Peptide 4 (K, W) Free Y Peptide 5 (K, Y) Peptide 6 (R, V) Peptide 7 (K, M, W)
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